Lab 2- Colostridium

Question 1

Which subspecies cause malignant oedema?

Answer 1

C. Septicum
C. Novyi
C. Hemolyticum
C. Sordelli

Question 2

Which subspecies cause blackleg?

Answer 2

C. Chauvoei

Question 3

Which subspecies cause human gas gangrene?

Answer 3

C. Perfringes A

C. Novyi A

Question 4

Which subspecies cause necrotic enteretitis in chicken?

Answer 4

C. Perfringes A+C

Question 5

Which subspecies cause lamb dysentery?

Answer 5

C. Perfringes B

Question 6

What subspecies cause necrotic enteretitis in pigs ?

Answer 6

C. Perfringes C

Question 7

Which subspecies cause pulpy kidney disease?

Answer 7

C. Perfringes D

Question 8

Which subspecies cause ulcerative enteretitis in chicken?

Answer 8

C. Colinum

Question 9

Which subspecies cause chronic enteretitis in piglets, foals and humans ?

Answer 9

C. Difficile

Question 10

Which subspecies cause tetanus?

Answer 10

C. Tetani

Question 11

Culture

Answer 11

Obligate anaerobic

Oxygen tolerance different

Question 12

Optimal temperature

Answer 12

37 degrees

Question 13

Biochemistry

Answer 13

Active metabolism
Most of them can split carbs (fermentation)
Exotoxic proteins

Question 14

Antigens

Answer 14

Complex

Close relationship, cannot be used in diagnostic work

Question 15

Resistance

Answer 15

Vegetative: medium
Spore: high resistance, can be boiled for 2 hours

Question 16

Subspecies grouping

Answer 16

Histotoxic = 
C. Septicum 
C. Novyi
C. Hemolyticum 
Cl. Histolyticum 
C. Sordelli 
C. Chauvoei
C. Perfringes A
C. Novyi A 
Enteropathogenic/enterotoxaemia=
C. Perfringes A, B, C, D
C. Colinum 
C. Difficile
Neurotoxic= 
C. Tetani 
C. Botulinum

Question 17

Which subspecies cause botulismus ?

Answer 17

C. Botulinum

Question 18

Trypsin causes activation of the toxins in which subspecies?

Answer 18

C. Botulinum

C. Perfringes A

Question 19

What subspecies cause flaccid paralysis (lameness)?

Answer 19

C. Botulinum

Question 20

Which subspecies cannot be cultivated in medium?(only living cells, like a virus)?

Answer 20

C. Piriforme

Question 21

Which subspecies cause tyzzer disease in dog, cat and foal ?

Answer 21

C. Piriforme

Question 22

Habitat

Answer 22

Soil, mud, water, gut

About 100 species

Question 23

Morphology

Answer 23

7-10 micro meter rods 
Bottle shaped flagella 
Spore exceeds the diameter of the cell 
Position of spore is typical = subterminal or terminal 
No capsule (except c. Perfringes)

Question 24

Staining

Answer 24

Gram positive