Lab 2- Colostridium Flashcards
Which subspecies cause malignant oedema?
C. Septicum
C. Novyi
C. Hemolyticum
C. Sordelli
Which subspecies cause blackleg?
C. Chauvoei
Which subspecies cause human gas gangrene?
C. Perfringes A
C. Novyi A
Which subspecies cause necrotic enteretitis in chicken?
C. Perfringes A+C
Which subspecies cause lamb dysentery?
C. Perfringes B
What subspecies cause necrotic enteretitis in pigs ?
C. Perfringes C
Which subspecies cause pulpy kidney disease?
C. Perfringes D
Which subspecies cause ulcerative enteretitis in chicken?
C. Colinum
Which subspecies cause chronic enteretitis in piglets, foals and humans ?
C. Difficile
Which subspecies cause tetanus?
C. Tetani
Culture
Obligate anaerobic
Oxygen tolerance different
Optimal temperature
37 degrees
Biochemistry
Active metabolism
Most of them can split carbs (fermentation)
Exotoxic proteins
Antigens
Complex
Close relationship, cannot be used in diagnostic work
Resistance
Vegetative: medium
Spore: high resistance, can be boiled for 2 hours
Subspecies grouping
Histotoxic = C. Septicum C. Novyi C. Hemolyticum Cl. Histolyticum C. Sordelli C. Chauvoei C. Perfringes A C. Novyi A Enteropathogenic/enterotoxaemia= C. Perfringes A, B, C, D C. Colinum C. Difficile Neurotoxic= C. Tetani C. Botulinum
Which subspecies cause botulismus ?
C. Botulinum
Trypsin causes activation of the toxins in which subspecies?
C. Botulinum
C. Perfringes A
What subspecies cause flaccid paralysis (lameness)?
C. Botulinum
Which subspecies cannot be cultivated in medium?(only living cells, like a virus)?
C. Piriforme
Which subspecies cause tyzzer disease in dog, cat and foal ?
C. Piriforme
Habitat
Soil, mud, water, gut
About 100 species
Morphology
7-10 micro meter rods Bottle shaped flagella Spore exceeds the diameter of the cell Position of spore is typical = subterminal or terminal No capsule (except c. Perfringes)
Staining
Gram positive
