Lab 7- Yersinia Flashcards
Species
Y. Pestis (black death-almost not existing anymore)
Y. Pseudotubercolosis (important in warm blood species)
Y. Enterocolitica (important in warm blood species)
Y. Ruckeri (fish)
Habitat y. Pseudotubercolosis
Intestine , water
Morphology y. Pseudotubercolosis
1-2 um coccoid rods
Flagella at 22 degrees
Staining
Gram negative
Bipolar staining
Culture y. Pseudotubercolosis
Not fastidious
Grow on low capacity media
Small colonies
Selective culture: enterobacteriaceae low capacity media
Biochem
Lactose-
Indole
-/+
Urease
+
Lactose
-
Mannitol
+
Methyl red
+
Voges-proskauer
-
H2S
-
Phenylalanine deaminase
-
Antigens y. Pseudotubercolosis
O antigen: 6 serogroups
H antigens : 5
Composite antigens
Pathogenicity y. Pseudotubercolosis
Rodents (rodentiosis)
Turkey, pheasant, quail (septicaemia, abscesses)
Cattle, swine, sheep (abortion)
Human ( inflammation of large intestine)
Habitat y. Enterocolitica
Intestine , water
Mainly in pigs
Morphology y. Enterocolitica
Middle sized rods
Gram negative
Culture y. Enterocolitica
Optimal temp 22 degrees
Selective culture: Na-oxylate+bile salts
Antigens y. Enterocolitica
O antigens: 34 groups
O9: cross reaction with brucella species
H antigens= 20
Pathogenicity y. Enterocolitica
Human = gastrointestinal infection, abscesses
Swine, rabbit: intestine, mesentherial lymph nodes, resembles tuberculosis
Biochem y. Ruckeri
Inactive
Pathogenicity y. Ruckeri
Trout septicaemia, kidney (red mouth disease in cat fish, infections of mucous membranes)
