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Bacteriology > Lab 6- Escherichia > Flashcards

Lab 6- Escherichia Flashcards

1
Q

Discovered by?

A

Theodor Escherich

Paediatrician

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2
Q

Habitat

A

Gut, aerobic, faculative anaerobic gut flora in mammals and birds

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3
Q

Morphology

A

2-3 um rod

Capsule, flagella, fimbria

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4
Q

Staining

A

Gram negative

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5
Q

Characteristics of flagella ?

A

Long protein tubes, tumbling motility

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6
Q

Culture

A

Selective and differential media

Low selectivity:

  • Drigalski (crystal violet, lactose, litmus)
  • Macconkey (crystal violet, bile salts, lactose, neutral red)
  • eosin, lactose, methylene blue

Medium selective:

  • brilliant green, lactose, phenol red
  • DC (deoxycholate-citrate, lactose, neutral red)

Seen with naked eye:

Capsulated (calf origin) = septicaemia in calf, Mucoid colonies

Haemolytic (swine origin)= on blood agar, pathogenic strains

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7
Q

Lactose ?

A

Positive

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8
Q

Mannitol

A

Positive

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9
Q

Indol production

A

Positive

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10
Q

Methyl red

A

Positive

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11
Q

Voges-proskauer

A

Negative

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12
Q

Citrate utilization

A

Negative

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13
Q

Urease

A

Negative

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14
Q

H2S

A

Negative

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15
Q

Phenylalanine deaminase

A

Negative

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16
Q

Antigens

A

O type = 174
K type= 80
F type= 19
H=57

Fimbria antigens most important

F1= common 
F2+3= human 
F4+6+18= pig
F5+17+41= calves 
F5=lamb
F7-16= UTI, human and poultry
17
Q

Resistance

A

Medium

Can survive for weeks in environment

18
Q

Pathogenicity

A

Saprophytes c faculative pathogenic

19
Q

Causes what in cattle?

A

Calf coli-diarrhea
Calf coli-septicaemia
Cow= mastitis

20
Q

Causes what in swine?

A

Coli-diarrhea in neonatal piglets

Weaned piglets= coli-diarrhea, oedema disease
Sow= mastitis, metritis

21
Q

Causes what in lamb/kid?

A

Coli-diarrhea (neonatal)

22
Q

Causes what in rabbit?

A

Diarrhea

23
Q

Causes what in poultry?

A

Day old chicken = septicaemia

Chicken= septicaemia, respiratory disease 
Older= lesions in organs (airsacculitis, peritonitis, coli-granulomatosis)
24
Q

Causes what in foal, dog, cat, Fox, nutria, chinchilla?

A

Coli-diarrhea, septicaemia

25
Q

Causes what in human ?

A

Neonatal enteretitis, septicaemia, urogential infections, enteritis, haemorrhagic enterocolitis

26
Q

Pathogenic e. Coli strains

A
  • Enteropathogenic or attaching/effacing (intimin)
  • Enterotoxic strains (enterotoxin)
  • Verotoxigenic
  • Neurotoxic
  • Enteroinvasive
  • Enteroadhensive-aggregative
  • Enteroheaemorrhagic
  • Septicaemic
27
Q

Characteristics of enteropathogenic?

A

Intimin required for colonization
Destruction of microvilli, atrophy and shedding of enterocytes

Maldig. + malabs.

Diarrhea in piglets, lambs and pups

28
Q

Characteristics of Enterotoxigenic

A 
F4 = pig
F5= calves, lambs 
LT= induce hypersecration - heat labile 
ST= reduces abs. - heat stabile

Diarrhea in neonatal piglets, calves and lambs
Post weaning diarrhea in pigs

29
Q

Characteristics of verotoxigenic

A

Bind to erythrocytes
Damage to vascular use in intestine and other locations

Oedema disease in weaned piglets
Haemorrhagic enterocolitis in calves
Post weaning diarrhea in pigs
Haemorrhagic colitis-haemolytic uraemic syndrome in human (lethal)

30
Q

Characteristics of necrotoxic

A

Bind to erythrocytes

Cytotoxic necrotizing factors

Damage to rbc and blood vessels

Haemorrhagic colitis in cattle
Enterocolitis in piglets and calves
Diarrhea in rabbits
Dysentery in horses

31
Q

characteristics of Septicaemic

A

Endotoxin effect
Invasion of blood streams

Septicaemia in birds
Colispeticaemia in calves, piglets, pups and chickens
Watery mouth in lambs
Arthritis, meningitis

32
Q

Characteristics of uropathogenic

A

Adhesions required for colonization
Endotoxins and exotoxins: local reactions

Cystitis in bitches

33
Q

Opportunistic strains

A

Mastitis in cattle and sows
Pyometra in bitches and queens

Metritis
Omphalitis in calves, lambs and chicks

Bacteriology (81 decks)

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