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Bacteriology > Lab 3- Staphylococcus > Flashcards

Lab 3- Staphylococcus Flashcards

1
Q

Habitat

A

Skin, mucous membrane, food, plant, soil and water

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2
Q

Morphology

A

Coccus

Bunch of grapes

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3
Q

Staining

A

Gram positive

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4
Q

Steps of gram staining

A 
Crystal violet 3-5 min 
Lugol solution 1-1,5 min 
96% alcohol, some drops 
Water 
Fuchsin 0,5-1 min 
Water
Drying
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5
Q

Culture

A

Nutrient agar, broth

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6
Q

Pigment

A

Golden, white

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7
Q

Hemolysis

A 
B hemolysis (sometimes double)
No hemolysis
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8
Q

Selective culture

A

10% NaCl-mannitol-phenol red agar

Må-tellurite-glycine-pyruvic acid - egg (Baird-Parker agar)

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9
Q

Biochem

A

Catalase +
Oxidase -
Fermentative

Extracellular enzymes:
Coagulase, fibrinolysin, hyaluronidase

Surface proteins: protein-A

Toxins:
haemolysis, leucocidins, enterotoxins, dermotoxin (exfolitative toxin)

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10
Q

Antigens

A

Complex

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11
Q

Resistance

A

Good

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12
Q

Pathogenicity

A

Local suppuration, abscesses, arthritis, mastitis, metritis, dermatitis, septicaemia

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13
Q

Species

Coagulase positive

A 
Extracellular enzymes 
Toxins 
Hemolysis
Mannitol + 
Facultative pathogens
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14
Q

Species

Coagulase negative

A 
Less extracellular enzymes 
Toxin prod. Not typical 
No hemolysis 
Mannitol -
Generally saprophytes, sometimes subclinical infections
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15
Q

Coagulase positive species:

A

S. Aureus subspecies aureus
S. Aureus subspecies anaerobius
S. Pseudointermedius
S. Intermedius

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16
Q

What species does s. Aureus subspecies aureus infect?

A

Cattle, swine, horse, sheep, dog and poultry
(Biotype A-E in different hosts)

MRSA: methicillin resistant s. Aureus

17
Q

What species does s. Aureus subspecies anaerobius target and what disease does it cause?

A

Sheep

Morel disease

18
Q

Target species and disease s. Pseudointermedius ?

A

Dog, cat

Dermatitis, otitis externa

19
Q

Target species s. Intermedius

A

Dog, horse, birds: mucous membranes

Saprophyte

20
Q

Coagulase negative species:

A 
S. Epidermis 
S. Haemolyticus
S. Hyicus 
S. Gallinarum 
S. Equorum 
S. Felis
21
Q

S. Epidermis target species and disease?

A

Horse and dog

Wound infection

22
Q

S. Hemolyticus found in ?

A

Milk

23
Q

S. Hyicus

A

A part of the strain is coagulase positive

Exudative epidermitis

24
Q

S. Gallinarum cause?

A

Dermatitis

25
Q

S. Equorum cause?

A

Dermatitis

26
Q

S. Felis cause?

A

Dermatitis

27
Q

Habitat Micrococcus?

A

Environment, mucous membranes, food, skin

28
Q

Morphology Micrococcus?

A

Coccus

29
Q

Biochem Micrococcus?

A

Glucose utilization is aerobic or missing

30
Q

Pathogenicity Micrococcus?

A

Saprophyte

Bacteriology (81 decks)

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