Lab 4-5 4-6 2-8 Role of Temp

Question 1

What microbe does MacConkey agar detect?

Answer 1

Enterobacteriacease to ferment lactose

Question 2

What does eosin methylene blue, EMB, contain?

Answer 2

Gelatin, lactose eosin Y and methylene blue

Question 3

What is EMB used for?

Answer 3

Fecal coliform

Question 4

What does MacConkey agar contain?

Answer 4

Lactose, bile salts, neural red, and crystal violet.

Question 5

What does the GasPak Anaerobic System do?

Answer 5

Creates anaerobic, microaerophilic, or CO2 enriched conditions

Question 6

What is in the GasPak?

Answer 6

sodium borohydride and sodium bicarbonate

Question 7

What is the catalyst in the GasPak?

Answer 7

Palladium

Question 8

MacConkey agar uses neural red dye. At what pH does it indicate?

Answer 8

0-6.8 pH. Organisms that ferment lower the pH.

Question 9

If you get good growth in eosin methylene blue, EMB, what is the presumptive ID?

Answer 9

Gram negative

Question 10

If you get purple metallic growth in eosin methylene blue, EMB, what is the presumptive ID?

Answer 10

Coliform

Question 11

If you get good growth in MacConkey agar, what is the presumptive ID?

Answer 11

Gram negative

Question 12

What is the name of the bacteria that grows between 0 degrees and 20 degrees C.?

Answer 12

Psychrophiles

Question 13

What is the name of the bacteria that grows between 20 degrees and 45 degrees C.?

Answer 13

Mesophiles

Question 14

What is the name of the bacteria that grows between 50 degrees and 60 degrees C?

Answer 14

Thermophiles