Lab 2-14 5-3 5-8 5-28 Flashcards
What is a disinfectant?
A germicide for use on surfaces.
What is a antiseptic?
A germicide for use on living tissue.
What is the name of the chemical germicide lab?
The Use-Dilution Test
What organisms does the Use-Dilution Test used on?
In class, Staph epidermidis and E. coli
What is a positive result for the Use-Dilution Test?
A positive result will be the surviving bacteria will make the broth turbid, indicating the germicide did not kill the bacteria.
What is the purpose of the Use-Dilution Test?
Test the effectiveness of germicides against bacteria.
What is the pH range does phenol red indicate?
Yellow below pH 6.8 Pink above pH 7.4 Red between pH 6.8-7.4
What is the name of the tube used to collect gas?
Durham tube
What is the name of the gas produced and what does it do to the pH?
Ammonia, NH3 Raises pH, turning phenol red pink
What is phenol red used for?
To differentiate enterobacteriacase from other Gram-negative rods.
What does deaminization mean?
Breaks amino acids becomes more basic, raises pH, making the broth pink.
What kind of broth is phenol red?
Differential
What does the fermintation of glucose begin with?
Pyruvate
What does anaerobic respiration do?
Transfer of electrons to an inorganic molecule other than oxygen.
What kind of broth is nitrate broth?
Undefined beef extract, peptone, and potassium nitrate.
