Ch 03 Microscope Flashcards
How is Robert Hooke’s invention related to Joseph Lister’s invention?
The compound lens and microscope
Was was van Leeuwenhoek’s invention?
Single lens 300X microscope
What is the advantage to oil immersion lenses?
Oil immersion lenses corrects the refractive index of glass which is a problem at high magnification.
Which lens is the occular and which is the objective?
Ocular: lens nearest the eye
Objective: lens nearest the slide
What is a smear?
A thin film of material containing the microbe to be studied under a microscope
What is a mordant?
A chemical added to the smear to intensify the stain
What devices focus light and electron microscopes?
Light - lenses
Electron - magnets
What is a chromophore?
Part of the molecule that reflects wavelengths back to the eye
What is Carolus Linnaeus contribution to science?
Developed two-part naming system
What is Robert Whitaker’s contribution to science?
Founded the five kindom system in which prokaryotes were placed in the prokaryote kindom
What is serology?
The study of blood serum and other bodily fluids
Which microscope is used to look at spirochetes?
Darkfield
What is the maximum resolving power and magnification of light and electron microscopes?
Light: 0.2 um, 2,000X
2D EM: 10 pm, 100,000X
3D EM: 10 nm, 10,000X
What is a simple stain?
Water or alcohol and dye
What can be used to improve the bonding between a simple stain and the specimen?
A modrant
In Two-Photon microscopy, what kind of dye is used?
Fluorescent dye
In Scanning Acoustic Microscopy, what is the medium used?
Sound waves
What kind of images do Scanning-tunneling Microscopy and Atomic Force Microscopy yeild?
3D
Name two differential stains
Gram stain: violet, iodine, alcohol decolorizer, red
Acid-Fast stain: acid-fast microbes for red,
non-acid fast methylene blue counterstain
What stain is used to make capsules visible?
Special stains
What stain is used to make endospore visible?
Special stains
A cell is stained because of __________?
ionic interaction
