Ch 10 Classification of Microorganisms Flashcards
What are the classic and moderns approaches to microorganisms?
Classic - behavior approach
Modern - gene approach
What does taxa mean?
A similar taxonomic group of any rank, such as a species, family, or class.
What does a viral species mean?
A population with similar characteristics.
What does serology mean?
The study of blood serum, especially with regard to the immune response.
What does phage mean?
A virus that parasitizes a bacterium by infecting it and reproducing inside it.
What are the two parameters that describe gel electrophoresis?
Smaller size travels farther
Brighter is higher concentration
How many different organisms are there?
1.5 million
What do all organisms have in common?
Cells surrounded by plasma membrane
Use ATP
Store genetic information as DNA
What is systematic phylogeny?
The study of evolutionary history of a group of organisms.
What are the three domains?
Eukarya-unicellular or multicelluar w nucleus
Bacteria-unicelluar wo nucleus
Archaea-unicellular wo nucleus
What are traits of archaea?
Prokaryotes
No peptidoglycan in cell walls
Live in extreme environments
Unusual metabolic processes
What are the traits of eukaryotes?
Protista
Fungi
Plantae
Animala
What are the traits of viruses?
Not composed of cells
Use the host to multiply
What percentage of microorganisms have been discovered?
1%
What are serological tests?
Slide agglutination
Lance field
ELISA
Western Blot
What is phage typing?
Phage typing is a method used for detecting single strains of bacteria. It is used to trace the source of outbreaks of infections.
What is flow cytomerty?
Flow cytometry is a technology that is used to analyse the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. Cell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths.
DNA base pair composition
Composition of guanine + cytosine.
What is special about fatty acid profile?
Consistent for a particular species
What is flow cytometry?
Flow cytometry is a technology that is used to analyse the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. Cell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths.
Pseudomonas
What is meant by DNA fingerprints?
Restriction enzymes
Comparison of a number of size and fragments
What is taq polymerase?
Taq polymerase is an enzyme that copies DNA.
What does nucleic acid hybridization mean?
Ability of DNA strands from one organism to hybridize to others
What does taxonomy mean?
The branch of science concerned with classification of organisms.
