Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Lab 2 questions > Flashcards

Lab 2 questions Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

mesophiles

A

20-40 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Thermophiles

A

60-80 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Psychrophiles

A

<20 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Psycrotrophs

A

special mesophiles; cold-tolerant mesophiles capable of growing at much colder conditions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

neutrophiles

A

6-8

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

acidophiles

A

less than 4

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

alkalinophiles

A

greater than 8

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

E coli

A

diarrheas, SSTI’s, UTIs, genetic research

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Salmonella enterica

A

food poisoning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Enterobacteriaceae Family common features

A

-gram negative bacilli
-facultative anaerobe
-oxidase negative
-catalase positive
-motile
-able to ferment glucose
-mesophilic

coliforms are enterobacteriaceae that can ferment lactose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

carbohydrate fermentations

A
  • Used to determine which carbohydrates an organism can ferment and which carbohydrate it cannot ferment
  • Phenol Red Broth is a carbohydrate free base medium
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Orange tube - sorbitol

A

did NOT ferment sorbitol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Yellow tube - sorbitol

A

Did ferment sorbitol made acidic wastes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Red tube - sorbitol

A

did NOT ferment sorbitol
DID digest proteins
released ammonia from amino acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Bubble in Durham tube

A

production of gas

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

enzyme is

A

protein that controls a chemical reaction

17
Q

catalyst is..

A

something that triggers a chemical reaction but that is not changed by the reaction

18
Q

endoenzymes

A

remain inside cells to perform their function

19
Q

exoenzymes

A

secreted from the cell to perform their functions

20
Q

oxidase performs…

A

the last step of the electron transport chain during cellular respiration

21
Q

catalase performs…

A

converts hydrogen peroxide to H20 and O2, it protects cells from hydrogen peroxide damage

22
Q

exoenzymes

A

-function outside of the cell that made it
-digestion of macromolecules
-ex: amylase, casease, penicilinase

23
Q

sometimes reagents …

A

need to be added to complete the test

24
Q

Amylase

-exo or endo
-grown on
-substrate
-product
-reagent
-results
-meaning

A

-exoenzyme
-grown on: starch agar
-substrate: starch
-product: glucose
-reagent: Iodine
-results: clear zone around the growth
-meaning: clear because starch has been digested ; starch digested because organism secreted the exoenzyme amylase

25
Q

Casease

-exo or endo
-grown on
-substrate
-product
-reagent
-results
-meaning

A

-ezoenzyme
-grown on: milk agar
-substrate: casein
-product: amino acids
-reagent: none
-results: clear zone around the growth
-meaning: clear because casein had been digested; casein digested because organism secreted the exoenzyme casease

26
Q

Gelatinase

-exo or endo
-grown on
-substrate
-product
-reagent
-results
-meaning

A

-exoenzyme
-grown on: nutrient gelatin
-substrate: gelatin
-product: amino acids
-reagent: none
-results: runny medium or solid medium
-meaning: runny if the gelatin has been digested; gelatin digested because organism secreted the exoenzyme gelatinase

27
Q

oxidase

-exo or endo
-grown on
-substrate
-product
-reagent
-results
-meaning

A

-endoenxyme
-grown on: any medium able to grow organism
-substrate: oxidase substrate
-product: reduced oxidase substrate
-reagent: oxidase substrate
-results: purply-blue
-meaning: Purply-blue indicates that the oxidase substrate has been reduced; oxidase substrate was reduced because the organism produced the endoenzyme oxidase

28
Q

Methyl Red test

A

-detects the production of stable acidic wastes from fermentation of carbohydrates
-simple pH
-Red- produced stable organic acids
-not red- did NOT produce stable organic acids

29
Q

Voges Proskauer test

A

-detects the production of unstable acidic wastes from fermentation of carbohydrates
-barrett’s A and B reagents
-red= organism produces unstable acid waste
-not red= did NOT produce unstable acid waste

30
Q

Citrate Utilization

A

can the organism use citrate (citric acid) as a carbon source?
-Blue= can use citrate
-Green= cannot use citrate

31
Q

IMViC tests

A

-indole production
-methyl red
-voges proskauer
-citrate utilization

32
Q

Indole production

A
  • digesting tryptophan by reoving the side cahin from this amino acid
  • DMACA
    -teal = produces indole
    -pink = does not produce indole`
33
Q

Listeria monocytognes

A
  • G- bacillus, psycotroph
  • diary and meat products
  • high affinity for fetal tissue
  • zero tolerance
34
Q

salmonella enterica

A
  • G- enterobacteriaceae
  • only if feces is present
  • food poisoning
  • zero tolerance
35
Q

staphylococcus aureus

A
  • G+ coccus
  • skin and respiratory tract
  • bad hygiene at deli counter
  • grows in food, producing toxins
  • food poisoning due to intoxication, not infection

Microbiology (19 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions