Lab 1 questions Flashcards
What are the flagella types?
-monotrichous (single @ 1 end)
-amphitrichous (Single @ both ends)
-lophotichous (multiple @ 1 end)
-peritrichous (multiple all over)
what are the 6 forms?
-punctiform
-circular
-filamentous
-irregular
-rhizoid
-spindle
what are the 6 elevations?
-flat
-raised
-convex
-pulvinate
-rhizoid
-spindle
what are the 6 margins?
-entire
-undulate
-lohate
-erose
-filamentous
-curled
Ubiquity
indicates that something is always present everywhere
general purpose
routine culturing / lots of different types can grow because it is rich / complex medium (soy bean flour)
what species lack a cell wall
mycoplasmas
G+ cell wall
thick layer of peptidoglycan
G- cell wall
thin layer of peptidoglycan and an outer membrane
what do gram stains find
size, shape(morphology), arrangement and stain
what is agar
a solidifying agent
chemically defined
chemical composition is known
chemically undefined (and ex.)
at least one ingredient is undefined
TSA - soy bean flour
minimal medium
basic nutrition for whatever you are trying to grow
rich
a complex medium that provides many (or all) of the basic building blocks
enriched
richer than rich. contains a type of blood
selective media
inhibit growth of certain types / selects growth of some crittes
differential media
does not inhibit the growth of any specific type of organisms / distinguish one type of organism from another type
Tryptic Soy Agar (TSA)
- Medium type
- Contains?
- Identify?
- Inhibit?
- Results?
- general purpose for routine cultures
- partially digested soybean flour
(undefined, complex, rich) - nothing too specific/colony characteristics
- none
- all growth
MAC - MacConkey Agar
- Medium type
- Contains?
- Identify?
- Inhibit?
- Results?
- a selective and differential medium useful for the identification of Enterobacteriaceae
- contains: crystal violet, bile salts, lactose and pH indicator
- Identify: causative agent of infections of enterobacteriaceae
- differentiates lactose-fermenting coliforms from the rest of the enterobacteriaceae family
- selects growth of Enterobacteriaceae
- results: growth or no growth? what color?
No growth: G+ or nonenteric bacteria
Growth: 1 or 2
1: Pink colonies: Lactose-fermenting Enterobacteriaceae (coliform)
2: Colorless colonies: enterobacteriaceae that does NOT ferment lactose
MSA - Mannitol Salt Agar
- selective and differential
- Contains: 7.5 % NaCl (S) and Mannitol (D)
- identify: pathogenic species of Staphylococcus
- inhibits almost all G+ bacilli, G- bacteria, members of streptococcus bacteria
- always growth of staphylococcus and micrococcus
-Growth or no growth / Yellow or red
No growth: G+ Bacilli / streptococci or G-
Growth: G+ staphylococcus or micrococcus 1 or 2
1: Yellow around growth: Pathogenic species of staphylococcus
2: medium stays red: nonpathogenic staphylococcus or micrococcus
Columbia CNA with 5% Sheeps Blood
- selective and differential
- contains : Colistin, Naladixic Acid
- Idenitfy Hemolysis - ability to damage red blood cells
- kills almost all G- bacteria
- rseults: growth or no growth; hemolysis or no hemolysis
Grwoth:
No hemolysis= gamma
partial hemolysis = alpha
complete hemolysis = beta
TSA with 5% sheeps blood / Blood Agar (BA)
- enriched
- nonselective but differential
- almost everything might grow
- damage to the RBC’s
Muddy green = alpha
no hemolysis but growth = gamma
clear zone around = beta
minimal medium
Provides basic nutrition for whatever you are trying to grow
Organism typically must make some (or all) of the basic building blocks
