Kandpal - DNA Repair Flashcards
What are the type of mutations of repair proteins?
Homologs of mutS and mutL
What type of damages of post replcation errors?
Depurination,Deamination, UV Damage (Pyrimidine dimers), Heat, Extreme pH, Radiations, substances in environment
What are the consequences of DNA damage
DNA will have an altered sequence during next round of replication.
Survival of a cell is dependant on?
Genetic stability
What is a transition error?
Substitution of a prryimidine with another pyrimidine or a purine with another purine.
What is transversion error?
Substitution of a pyrimidine with a purine or vice versa
What is frameshift error?
Due to addition/deletion of bases (codon)
What is the 1st level of replair during replication.
Proofreading - 3’ to 5’ exonuclease activity of DNA polymerase.
What removes mismatched nucleotides?
The 3’ to 5’ exonuclease during proofreading
What happens when replication errors are missed by proofreading activity?
Distortion in the DNA helix is recognized and repaired by strand directed mismatch repair
For a mismatched E. Coli, both stands are methylated at what junction?
Adenine
What recognizes mismatched bases?
MutS
What scans the DNA where the “nick” to determine the newly replicated DNA?
MutL
What is the percentage of colon cancer cases that are familial (run in the family)?
10 to 15%
What are the 2 familial colon cancers?
HNPCC (Hereditary non-polyposis colon cancer) and FAP (Familial adenomatous polyposis)