Kandpal - DNA Repair Flashcards

1
Q

What are the type of mutations of repair proteins?

A

Homologs of mutS and mutL

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2
Q

What type of damages of post replcation errors?

A

Depurination,Deamination, UV Damage (Pyrimidine dimers), Heat, Extreme pH, Radiations, substances in environment

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3
Q

What are the consequences of DNA damage

A

DNA will have an altered sequence during next round of replication.

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4
Q

Survival of a cell is dependant on?

A

Genetic stability

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5
Q

What is a transition error?

A

Substitution of a prryimidine with another pyrimidine or a purine with another purine.

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6
Q

What is transversion error?

A

Substitution of a pyrimidine with a purine or vice versa

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7
Q

What is frameshift error?

A

Due to addition/deletion of bases (codon)

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8
Q

What is the 1st level of replair during replication.

A

Proofreading - 3’ to 5’ exonuclease activity of DNA polymerase.

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9
Q

What removes mismatched nucleotides?

A

The 3’ to 5’ exonuclease during proofreading

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10
Q

What happens when replication errors are missed by proofreading activity?

A

Distortion in the DNA helix is recognized and repaired by strand directed mismatch repair

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11
Q

For a mismatched E. Coli, both stands are methylated at what junction?

A

Adenine

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12
Q

What recognizes mismatched bases?

A

MutS

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13
Q

What scans the DNA where the “nick” to determine the newly replicated DNA?

A

MutL

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14
Q

What is the percentage of colon cancer cases that are familial (run in the family)?

A

10 to 15%

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15
Q

What are the 2 familial colon cancers?

A

HNPCC (Hereditary non-polyposis colon cancer) and FAP (Familial adenomatous polyposis)

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16
Q

When you remove a purine from a nucleotide, what is left over?

A

A base-less sugar and cytosine will convert into a uracil.

17
Q

Can thymine be deamination?

A

NO. It has no amino group

18
Q

What does 5-methyl cytosine can be deaminated?

A

Yes. Into thymine

19
Q

Can you create a deletion in deamination?

A

No only in depurination.

20
Q

What is base excision repair?

A

Specific base is removed and repaired

21
Q

What is nucleotide excision repair?

A

A stretch of DNA is removed and a new strand with the correct base is synthesized.

22
Q

What is the Yeast repair names for excision repair?

A

RAD3

23
Q

What is the Yeast repair for post-replication repair?

A

RAD 6

24
Q

What is the Yeast repair for recombination -mediated repair?

A

RAD52

25
Q

DNA or RNA, what is RAD3 part _____ polymerase?

A

RNA

26
Q

When does double strand breaks occur?

A

During Recombination and Ionizing radition

27
Q

What 2 processes repair double strand breaks?

A

Homologous end joining (HEJ) and Non Homologous end joning (NHEJ)

28
Q

Since BRCA1 and BRCA2 are defects in double stranded break repair, what do BRCA1 and BRCA2 stand for?

A

Breast Cancer gener 1 and gene 2. Predispose to both Breast and Ovarian cancer.

29
Q

What is the percentage of breast cancer cases that are familial?

A

5%

30
Q

What is the susceptibility to breast cancer?

A

Autosomal dominant

31
Q

What is Werner Syndrome?

A

An Autosomal recessive disease. Causes cataracts, changing of skin conditions, short stature, premature graying, thinning of hair.

32
Q

What is Bloom syndrome?

A

Autosomal recessive. Stunted growth, sunlight sensitivity, chromosome breakage, immunologic deficiency, infertility, increased risk for neoplasia. DOES NOT INVOLVE 3’-5’ EXONUCLEASE ACTIVITY.