Brar - Enzymes, Enzyme Kinetics Flashcards
What do and don’t enzymes do?
Catalyze specific biochemical reactions
DO NOT create new reactions.
List 4 general areas where enzymes are needed as catalysts.
Mitochondrion, cytosolic, lysosomal, and nuclear areas.
How do enzymes catalyze reactions?
They are specific and reduce the activation energy required for the reaction to occur.
Can enzymes be used more than once?
Yes, they are not destroyed and return to their original state after rxn.
What occurs in an enzyme’s active site?
an enzyme-substrate complex is formed and the site contains functional groups that help in rxns
What are the 2 substrate binding models?
Lock and key model
Induced fit model
Describe the Lock and key model.
fixed and rigid model; small differences in structure can prevent proper binding
Describe the Induced Fit model.
flexible 3-D structure that undergoes conformational change when substrate binds and exposes more residues.
What is the transition state complex?
maximum energy level reached during a rxn; it is unstable and decomposes to products
What is activation energy?
the difference in energy between the substrate and the transition state complex
Which amino acid is one of the more important functional groups of an active site?
Serine-OH in proteases
What effects does pH have on enzyme catalysis?
it can denature the enzyme, decrease its function the farther away from it’s ideal pH level, or change its function based on the pH level
What are 2 mechanism based inhibitors?
Covalent inhibitors and Transition State Analogs
How do covalent inhibitors work?
covalently bind to residues in active site and renders enzyme useless or dead
Is a covalent inhibition reversible?
No, it is irreversible.
