History Flashcards
What is Microbiology?
Study of organisms and agents too small to be seen by the naked eye
Longest known bacterium
Epulopiscium, with paramecium
“Sulfur Pearl of Namibia”
Thiomargarita namibiensis
New definition of microbiology
Study of organcisms that can exist as a single cell, contain nucleic acid genome, and capable of replicating that genome
Importance of Microorganism
First living organism Live everywhere life is possible More in number than others Global ecosystem depends on their activities Influence human society
Basic Microbiology
Bacteria Archaea Algae Fungi Protozoa Viruses
Bacteria. Uni or Multi?
Unicellular
Bacteria. Eukaryote or prokaryote?
Prokaryote
Found in cell walls of bacteria
Peptidoglycan
Division of bacteria
Binary Fission
May be possessed by bacteria
Flagella
Pneumonia-causing bacteria
Haemophilus influenzae
Archae. Eukaryote or prokaryote?
Prokaryote
Lacking in archaea cell wall
Peptidoglycan
Found in extreme environments
Archaea
Adapted to high salt concentrations
Halophiles
Methane producers
Methanogens
Adapted to high temperature
Thermophiles
Adapted to high pressure
Barophiles
Mushroom, yeasts, molds
Fungi
Fungi. Eukaryote or prokaryote?
Eukaryote
Fungi. Uni or Multi?
Could be both
Found in true fungi’s cell walls
Chitin
Found in molds
Mycelia
Long filaments in mycelia
Hyphae
Cottony growth found on bread and fruit
Mold mycelia
How does fungi obtain nutrients?
Absorbing organic material from their environment
Common bread mold
Mucor sp.
Protozoa. Uni or Multi?
Unicellular
Protozoa. Eukaryote or Prokaryote?
Eukaryote
Protoza are moved by
Pseudopods
Flagella
Cilia
Protozoa obtain food by
Absorption
Ingestion
Protozoa reproduce sexually or asexually?
Both sexual and asexual
Example of protozoa
Amoeba
Algae. Uni or Multi?
May be both
Algae. Eukaryote or Prokaryote?
Eukaryote
Algae reproduce sexually or asexually?
Both sexual or asexual
Algae cell wall composition
Cellulose (like plants)
Algae obtain nourishment by
Photosynthesis
Algae produce
Oxygen and carbohydrates used by other organisms
Algae are found in
Freshwater
Saltwater
Soil
Plants
Example of algae
Volvox sp.
Noncellular entities that are parasites of cells
Virus
Virus consists of
Nucleic acid core (DNA or RNA) surrounded by protein coat
May surround virus coat
Envelope
Virus reproduction
Reproduce only by using cellular machinery of other organisms
Virus. Living or Nonliving?
Nonliving because they are inert outside living hosts
Rabies virus
Rhabdovirus
Other virus examples
Zika virus
Dengue virus
Applied Microbiology
Medical Microbiology Immunology Public Health Microbiology Food and Dairy Microbiology Industrial Agricultural Microbial Ecology
Nomenclature of Microbiology. Established by
Carolus Linnaeus (1735)
Scientific name are from
Latin words
Scientific names composition
Genus and species name; underlined or italicized
Staphylococcus aureau. Staphylo
Clustered arrangement of cells
Staphylococcus aureau. Coccus
Sphere-like shapes
Staphylococcus aureau. Aureus
Golden, color of many colonies of this bacterium
Founded by Theodor Escherich
Escherichia coli
Coli - found in colon
Made the first simple light microscope (3-10x)
Zacharias Jansen (1597)
Made the simple compound microscope (30x)
Robert Hooke - 1665
Laid foundation of cell theory
Robert Hooke - 1665
First to oberserve microorganisms
“wee animalcules”
Anton van Leeuwenhoek (1670)
The conflict
Spontaneous generation theory
Vs
Biogensis theory
Life from non-living matter
Vital source required
Spontaneous generation theory
Life begets life
Biogenesis theory
Negated spontaneous generation. Maggots appear on decaying meat only when flies are able to lay eggs on the meat
Francesco Redi (1668)
Strengthened spontaneous generation. Microorganisms arise spontaneously from heated nutrient broth
John Needham - 1745
Suggested that Needham’s result were due to microorganisms entering the broth
Lazzaro Spallanzani - 1765
SG supporters claim
Air was required for the vital force to work; vital force was destroyed by heat
Passed air through strong acids but no microorganisms formed
Franz Schule
Passed air through red-hot tubes but no microorganisms formed
Theodor Schwann
SG supporters claim
Strong acids and heat altered the air so it cannot support microbial growth
Filtered air through sterile cotton wool but no microbial growth found
Georg Friedrich Schroder
Theodor von Dusch
1850
Introduced the concept of biogenesis
Life begets life
Rudolf Virchom - 1858
Father of modern microbiology
Introduced pastuerization
Louis Pastuer - 1861
Demonstrated that dust carries microorganisms
Provided evidenxe for the existence of heat-resistant bacteria
John Tyndall (1820-1893)
Discovered the existence of heat-resistant bacterial endospores
Ferdinand Cohn
Diseases are caused by specific agents called germs
Germ Theory of Disease
Said that disease was caused by invisible living creatures
Grolamo Fracastoro (1478-1553)
Showed that the pebrine disease of silkworms was caused by a protozoan
Louis Pasteur
Showed that a disease of silkworms was caused by a fungus
Agostino Bassi (1773-1856)
Demonstrated that the great potato blight of Ireland was caused by a Fungus
Miles Joseph Berkeley (ca. 1845)
Showed that smut and rust fungus caused cereal crop diseases
Heinrich Anton de Bary (1853)
Asepsis in obstetrical wards to prevent the transmission of childbed fever from patient to patient
Ignaz Philipp Semmelweis (1861)
“Father of Antiseptic Urgery”
Joseph Lister (1867)
Established relationship between Bacillus anthracis and anthrax
Robert Koch
Koch’s Postulates
Used a vaccination procedure to protect individuals from smallpox
Edward Jenner (ca. 1798)
Last smallpox case
Somalia in 1977
Year smallpox was declared by WHO as eradicated
1979
Developed porcelain bacterial filter
Isolated the first viruses studied
Charles Chamberlan (1851-1908)
Developed 606th compound SALVARSAN (inorganic arsenical to treat syphilis)
Paul Ehrlich
Dicovered “miracle drug” penicillin from Penicillium
Alexander Fleming
Developed pastuerization to preserve wine
Louis Pasteur
Cell-free fermentation
Extract from yeast cells
Eduard Buchner