Bacterial Genetics Flashcards
Science concerned with hereditary and variation
Genetics
Most common bacterial reproduction and important in bacterial growth cycle
Asexual Mode
Exchange of genes between 2 DNA molecules to form combination of genes in a chromose
Gene recombination
Change in the base sequence of cell/organism which may occur naturally or through induction by agents
Mutations
Bacteral reproduction where bacteria are not differentiated to somatic or gametic cells
Sexual Mode
2 functional/mating types
Donor (male)
Recipient (female)
Involves a unidirectional transfer of a portion of DNA from donor or recipient
Sexual Mode
Gene transfer =
Genetic recombination
Genetic recombination =
Changes
Changes =
Variations
Metods of genetic transfer
Conjugation
Transduction
Transformation
Protoplast Fusion
Transfer of genetic information via cell-to-cell contact
Conjugation
Extra chromosal, independently replicating DNA found in bacteria and yeasts
Plasmids
Plasmid that can exist with or without being integrated into the host’s chromosome
Episome
Have genes for sex pili (transfer gene)
Conjugative plasmids
Fertility factor - genes for cell attachment, genes for transfer
F factor
Contains extra chromosomal F factor (donor)
F+
No F factor (recipient)
F-
High frequency of recombination
F factor integrated into bacterial chromosme
Donor
Hfr
Transfer of bacterial genes by viruses (most common mechanism)
Transduction
Two kinds of transduction
Generalized
Specialized / Restricted
Occurs during the lytic cycle of virulent and temperate viruses
Host DNA derived from any portion of the host genome becomes a part of the DNA of the virion in place of the virus’ genome
Generalized Transduction
Occurs only in some temperate viruses
Involves transfer of specific region of the host chromosome into the virus replacing some of the viral genes
Specialized / Restricted Transduction
Uptake by a cell of a naked DNA molecule or fragment from the medium and the incorporation of this molecule into the recipient chromosome
Contact with competent cell (able to take up DNA)
Transformation
Used in microorganisms with NO known conjugation system
Enzymes are used to digest cell wall to produce protoplasts
Protoplast Fusion
Industrially important aerobic organisms that convert ethanol into acetic acid (vinegar)
Acetobacter
Gluconobacter
Obligate intracellular parasite transmitted to humans by insect and tick bites
Responsible for a number of diseases known as the spotted fever group
Rickettsia sp.
Causes endemic murine typhus
R. typhi
Rickettsia-like bacteria that live obligately within white blood cells
Trabsmitted by ticks to humans and cause ehrlichiosis
Ehrlichia sp.
Freshwater microorganism
Caulobacter
Hyphomicrobium
Agriculturally important bacteria
Rhizobium
Bradyrhizobium
Agrobacterium
Small nonmotile coccobacilli
Obligate parasites and cause brucellosis
Brucella sp.
Genera of nitrifying bacteria that are of great importance to the environment and to agriculture
Nitrobacter
Nitrosomonas
Most common infectious bacterial genus in the world
Live only inside the cells of their hosts
Wolbachia
Found mainly in freshwater
Spirillum
Pseudomonas-like, aerobic
Burkholderia
Best known species of Burkholderia and has an extraordinary nutritional spectrum and is capable of degrading more than 100 different organic molecules. These bacteria may actually grow in disinfectant solutions.
Burkholderia cepacia
Aerobic
Neisseria
Causative agent of gonorrhoea
Neisseria gonorrhoeae