Enzymes Flashcards

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1
Q

What is an Enzyme?

A

Enzymes are proteins that help speed up metabolism, or the chemical reactions in our bodies.
It is a catalyst

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1
Q

What do enzymes consist of?

A

Amino acids- end in -ase, ex: amylase,maltase,sucrase

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2
Q

What is the function/goal of the enzyme?

A

To speed up the rate. RXN

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3
Q

What is activation energy?

A

The minimum amount of energy to start the rate RXN

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4
Q

What makes up an enzyme?

A

Weak hydrogen bonds, and amino acids

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5
Q

What does the substrate do?

A

It attaches to the enzymes active site

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6
Q

What are reactants?

A

a substance that takes part in and undergoes change during a reaction. On the left hand side.

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7
Q

What are products?

A

Products are the end result of the reaction and are written on the right-hand side of the equation.

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8
Q

What group do cofactors and coenzymes live in?

A

Prosthetic groups, they increase the rate of enzyme catalyzed reactions

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9
Q

What are coenzymes?

A

organic molecules that are needed to attach to certain enzymes to carry out the chemical reaction

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10
Q

How do coenzymes work?

A

They attach to the enzyme and allow substrate to bind and the reaction occurs.

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11
Q

What is an example of a coenzyme?

A

Vitamin B

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12
Q

What type of substance is cofactors classified as?

A

The are classified as metalion substances.

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13
Q

Example of cofactors?

A

Non-protein and organic ions. (lead)

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14
Q

What does hemoglobin do?

A

It is a protein that carries oxygen in blood cells.

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15
Q

What do inhibitors do?

A

Slows the rate of enzyme catalyzed reactions

16
Q

Irreversible inhibitors

A

Forms a covalent bond with the enzyme at its active site therefore permanently blocking the subtrate.

17
Q

Example of irreversible inhibitor?

A

Pb – lead —-> lead poisoning affects 3rd structure of the neurological protein.

18
Q

Reversible inhibitor

A

Forms a temporary bond with the enzyme therefore slows the reaction rate while the inhibitor is bound to the substrate.

19
Q

How can you overcome the effects of the reversible inhibitor?

A

By adding more substrate.

20
Q

Example of rev. inhibitor ?

A

CO2 binds to hemoglobin

21
Q

What does irreversible inhibitor do to the body?

A

It denatures the cells in the body

22
Q

Competitive inhibitors

A

Inhibitors with a similar size and shape to a substarte.

23
Q

What do Competitive inhibitors do?

A

They compete with the substrate to bind with the active site

24
Q

Example of Competitive inhibitors?

A

Antibiotics —–> blocks enzymes needed for reproduction

25
Q

What does the active site do?

A

Maintains homeostasis and binds with the catalyst

26
Q

what is pH

A

measure of the concentration of hydrogen ions in a soloution

27
Q

What is the optimum temperature for an enzyme to function and max rate?

A

37 C and 98.6 F

28
Q

What happens if you go beyond pH opt.

A

Enzymes starts to denature and breaks R group.

29
Q

More heat =________?

A

Denatures

30
Q

Substrate concentration

A

There is a fixed amount of enzyme but increasing amount of substrate.

31
Q

Enzyme concentration

A

There is a fixed amount of substrate but an increasing amount of enzyme

32
Q

Allosteric site

A

site where the allosteric inhibitor binds to the enzyme

33
Q

Allosteric inhibitor

A

Inhibitor that is non competitive, does not compete with substrate but binds to enzymes allosteric site.

34
Q

What happens if body temp. goes beyond the optimum?

A

Enzymes starts to denature

35
Q

Can enzymes that are denatured, renature?

A

Yes, only sometimes, not always possible