Biotechnology Flashcards

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1
Q

Vector

A

Host DNA or Host organism

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2
Q

Recombinant DNA

A

Bacteria plasmid + gene of interest

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3
Q

Restriction Enzyme

A

Enzyme that cuts a specific DNA site

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4
Q

Palindrome

A

Sequence that is the same backwards - Example:
Naman

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5
Q

Sticky Ends

A

Jagged exposed ends with exposed nucleotides

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6
Q

Blunt Ends

A

Ends with no jaggedness (straight)

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7
Q

Agriculture

A

Insert gene to prevent pest from eating it, no need for insecticides if done

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8
Q

Gene Therapy

A

Normal gene get place into cells that have defective genes

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9
Q

How do transgenic species reproduce?

A

Binary Fission (Asexual)

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10
Q

Plasmid

A

Circular pieces of DNA

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11
Q

Biotech.

A

Use of organism DNA to make products

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12
Q

Ligase

A

Enzyme used to join 2 DNA sources together

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13
Q

How to make clone?

A
  1. Remove nucleus from skin cell
  2. Remove nucleus from egg
  3. Combine the Skin cell nucleus and the empty egg cell
  4. Divide by mitosis
  5. Implant into uterus.
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14
Q

Sequence of jagged cuts

A

GAATTC

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15
Q

Whats RFLP?

A

Restriction fragment length polymorphism- Junk
Fragments of cut DNA

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16
Q

What do VNTRs do?

A

Makes different sizes of cut DNA pieces from restriction enzyme.