DNA Replication Flashcards
hehe milk
How long are Okazaki fragments
~200 nucleotides long
What direction are the deoxyribose sugars facing on the leading strand
They are pointed downwards
What are the bonds between the phosphates and sugars
Covalent bonds
What are the bonds between the nitrogenous bases
h-bonds
Why is Chargaff’s base pairing rule true
because purines can only bond with pyrimidines so for every purine, there must be 1 pyrimidine. So by this logic, % of Guanine = % of cytosine (not the same for adenine, thymine and uracil)
What is Chargaff’s base pairing rule
% of purines = % or pyrimidines
what is the structure of ribose
OH
|
ch2
|
C–O–C
\ /
C—–C
| |
OH OH
what is deoxyribose structure
OH
|
ch2
|
C–O–C
\ /
C—–C
| |
OH H
What prevents DNA from connecting back together
Single binding protein
What are the 4 bases
Adenine, Guanine, Cytosine, Thymine, (Uracil in RNA)
How does DNA Polymerase 3 start binding base pairs
RNA Primer is added and primes the leading stand once and allows for DNA Polymerase to find the complimentary base pairs.
What does topoisomerase do
it uncoils DNA
What is nucleotide triphosphate
an atp like molecule that binds to RNA primer’s 3’ end
What is the growth change between Leading stand vs Lagging stand
Leading strand has continuous growth vs lagging stand grows in chunks (Okazaki fragments)
Where do the bases come from for DNA Polymerase 3
they are free floating
