ENTEROBACTERIACEAE - SALMONELLA Flashcards
Salmonella is isolated and described as ?
Hog cholera bacillus (Salmonella cholerasuis)
Salmonella is named after?
D.A. Salmon in 1885
Give the 6 classifications of Genus Salmonella.
I. Typical Salmonellae
* S. cholerasuis
* S. enteritidis
* S. galinnarum
* S. typhi
* S. typhimurium
II. Atypical salmonellae
* S. salamae
III. S. arizonae and related organisms
IV. S. houtinae and related organisms
V. S bongor and related organisms
Describe the morphology of Salmonella.
- Gram (-)
- Non-capsulated short rods
- Peritrichous flagella with fimbriae
Give some examples of species of Salmonella that is non-flagellated.
- S. gallinarum
- S. pullorum
Classify Genus Salmonella according to their oxygen requirement.
Aerobic and facultative anaerobic
Give some examples of agar that can be used to culture Salmonella.
- BGA
- MacConkey agar
- Bismuth sulfite agar
Salmonella produces gases from _____ but can’t ferment ______.
glucose; lactose
What temp. does Salmonella grow?
37 C; 43 C
What is the only specie of Salmonella that contains Vi antigen ?
S. typhi; cause of typhoid in humans
What are the antigens present in Salmonella ?
- O antigen
- H antigen
- Vi
Variation in ____ antigen can change the colony variation from smooth (S) to rough which can result to loss of _____ antigen and sometimes virulence
O
What is the main mode of transmission of Salmonella ?
Feco-oral route
What specie of Salmonella is universally distributed ?
S. typhimurium
Enumerate the host-adapted and non-host adapted salmonellae.
HOST ADAPTED
* S. abortus equi
* S. abortus ovis
* S. cholerasuis
* S. dublin
* S. gallinarum S
* S. paratyphi (A, C)
* S. pullorum
* S. typhi
NON-HOST ADAPTED
* S. anatum
* S. derby
* S. newport
* S. tennessee
* S. typhimurium
Older animals are more susceptible to salmonellosis than younger ones. True or False?
False
Salmonellae can be killed at what temp and duration?
56 C for 10 to 20 minutes (except S. senftenberg)
Salmonellosis starts at _____ infection then reaches the bloodstream.
enteric
What is the first step of the pathogenesis of Salmonellae ?
Colonization of distal small intestine and colon
Enumerate the 3 phases of the pathogenesis of Salmonellae.
- Colonization of the intestine
- Invasion of the intestinal epithelium
- Stimulation of fluid exsorption
(please refer on the ppt for principles of the 3 phases)
In diseases of cattle, what species of Salmonellae are the most important?
- S. dublin (host-adapted)
- S. typhimurium (can be found throughout the world)
Name the species of Salmonella that affects poultry with the given data:
- Highly fatal to young chicks during first few days of life
- Transmission is transovarial
- Infected eggs may fail to hatch or hatch into infected chicks which if stressed may
become ill with diarrhea and septicemia - Transmission via incubator -> inhalation
- Chicks huddle near heat source, do not eat, appear sleepy, have diarrhea and die within
a few hours
12 - Meningoencephalitis
- Caseous areas in lungs, gizzard and heart muscle
S. pullorum
Name the species of Salmonella that affects poultry with the given data:
- Fowl typhoid in chickens and turkeys
- Cross-reacts with S. pullorum
- Vertical transmission
- Horizontal transmission through feces, broken eggs and by tick Argas persicus
- Affects adult birds
- Weakness, drowsiness, wing drooping, , hyperexcitability, paresis, diarrhea
- Rapidly developing anemia and leukocytosis
- birds found dead, hemorrhages in subcutis and parenchymatous organs, multiple small
necrosis in liver and heart and enlarged spleen
S. gallinarum
Name the species of Salmonella that affects poultry with the given data:
- Paratyphoid
- Enteritis, diarrhea and septicemia
- Can localize in ovaries and be transmitted via the egg
- Common in squabs, die soon after hatching or develop swollen wing joints and unable
to fly (megrims) -> gelatinous exudate in joint capsule - Infection in the ovary
S. typhimurium
What are the immunity methods in poultry against Salmonella ?
- Cell mediated but facultative intracellular parasite
- Live salmonella vaccines, attenuated, bacterins
What are the diagnostic methods for Salmonella ?
- Serology - agglutination test, antiglobulin test, ELISA
S. enterica is formerly known as?
S. cholerasuis