ENTEROBACTERIACEAE - KLEBSIELLA

Question 1

Klebsiella has how many species?

Answer 1

4

Question 2

Among the species of Klebsiella, which is pathogenic for animals?

Answer 2

Klebsiella pneumoniae

Question 3

Klebsiella pneumoniae is an opportunistic invader of ?

Answer 3

1. Reproductive tract of mares
2. Bovine mammary gland
3. Urinary tract of bitches

Question 4

What type of infection does Klebsiella normally induce?

Answer 4

Nosocomial infection in human and animal patients - due to its ability to acquire multiple antibiotic resistance and to spread in hospital environments.

Question 5

What is the other name of Kelbsiella pneumoiae?

Answer 5

Friedlander’s bacillus

Question 6

Describe the morphology of Klebsiella pneumoniae.

Answer 6

Gram negative, non-sporeforming, heavily encapsulated rod 0.3 to 1.0 um x 0.6 to 6.0 um, non-flagellated

Question 7

Describe the colonies of K. pneumoniae.

Answer 7

Colonies are moist, spreading, glistening and very viscid

Question 8

Capsule production of K. pneumoniae is enhanced by ?

Answer 8

Carbohydrate
** Carbohydrate medium

Question 9

K. pneumoniae is oxidase positive. True or False?

Answer 9

False

Question 10

Classify K. pneumonaie according to its oxygen requirement.

Answer 10

Facultative anaerobe

Question 11

K. pneumoniae uses tartrate and malonate. True or False?

Answer 11

True

Question 12

K. pneumoniae produces H2S. True or False?

Answer 12

True

Question 13

K. pneumoniae produces urease. True or False?

Answer 13

True

Question 14

K. pneuomoniae is positive in MR test but negative in VP test. True or False?

Answer 14

False, other way around

Question 15

Lactose positivity of K. pneuomoniae is on the genome or on the plasmid?

Answer 15

Plasmid

Question 16

Give the antigens of K. pneumoniae and its quantities.

Answer 16

1. 82 capsular types
2. 11 O antigens
3. K - for serologic typing only

Question 17

It is a heat-stable polysaccharide composed of one-charged monosaccharide usually glucuronic acid and two or more sugars galactose, fucose, mannose, D-glucose or L-rhamnose.

Answer 17

K antigen

Question 18

K. pneumoniae is _____ commensal but also can be found in the environmet.

Answer 18

intestinal

Question 19

What is the important predisposing factor of K. pneumoniae?

Answer 19

Usage of antibiotics

Question 20

Invasive strains of K. pneumoniae are resistant to ?

Answer 20

Serum bactericidal factors

Question 21

Mares carry the organism, as normal flora, in ? (Hint: body parts)

Answer 21

1. Vestibule
2. Urethra
3. Clitoris

Question 22

Opportunistic invasion of the organism result in ?

Answer 22

1. Vaginal discharge
2. Metritis
3. Infertility
4. Abortion

Question 23

Stallion can transmit from mare to mare and they can also be a reservoir of infection. True or False?

Answer 23

False, usually not reservoir

Question 24

The organism is an important component of etiology of ____ ____ in cattle.

Answer 24

coliform mastitis

Question 25

Give the diseases induced by K. pneuomoniae infection.

Answer 25

1. Vaginal discharge, metritis, infertility, abortion 2. Acute or peracute form of mastitis 3. Hyperemia; edema and swelling of the udder and milk secretion reduced and altered to **serous fluid** 4. High fever initially which may subside in a few hours if animal develops often fatal endotoxic shock 5. Mastitis in sows and pneumonia in goats

Question 26

What are the forms of **immunity** against K. pneuomoniae ?

Answer 26

* Little known about protective immunity to K pneumoniae * Only strains resistant to serum bactericidal factors invasive * Serum antibody measured by capsular swelling technique

Question 27

What are the **diagnostic methods** for the mentioned organism?

Answer 27

1. Heavily encapsulated appearance I smears 2. Mucoid colonies on agar 3. Tampons left in place for 10 minutes more effective in detecting uterine infections in mares than simple swabbing

Question 28

K. pneumoniae is **resistant** to what antibiotics?

Answer 28

1. Ampicillin 2. Carbenicillin

Question 29

K. pneumoniae is **maybe susceptible** to antimicrobials against _______.

Answer 29

E. coli

Question 30

____ is **effectve** in treament of canine urinary tract infections.

Answer 30

Cephalexin