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BURKHOLDERIA Flashcards

1
Q

Other names of Burkholderia pseudomallei

A
  1. Bacillus of Whitmore
  2. Malleomyces pseudomallei
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2
Q

A glanders-like disease caused by B. pseudomallei that is first described by Whitmore and Krishnaswami.

A

Meliodosis

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3
Q

B. pseudomallei is faculatative intracellular parasite. True or False?

A

True

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4
Q

Give the morphology and staining features of B. pseudomallei.

A
  • Gram negative with peritrichous flagella
  • May show bipolar staining
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5
Q

B. pseudomallei is fastidious. True or False?

A

False, grows on simple media

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6
Q

Describe the colonies of B. pseudomallei.

A

Colonies are rough to mucoid and cream-colored to orange

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7
Q

Acid production of B. pseudomallei can be derived from what sugars and alcohol?

A
  1. Glucose
  2. Sucrose
  3. Maltose
  4. Mannitol
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8
Q

B. pseudomallei produces oxidase. True or False?

A

True

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9
Q

B. pseudomallei produces pyoverdine. True or False?

A

False

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10
Q

B. pseudomallei is widely distributed in?

A

Soil, water, and feces

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11
Q

Infection of B. pseudomallei is by ?

A

Inhalation or via wound

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12
Q

B. pseudomallei is frequently transmitted among animals and humans. True or False?

A

False, rare

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13
Q

Meliodosis can be observed in what animals?

A
  1. Cats
  2. Dogs
  3. Pigs
  4. Goats
  5. Sheep
  6. Horses
  7. Dolphin
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14
Q

Describe the characteristic lesions induced by B. pseudomallei.

A

Small caseous nodule (similar to tuberculosis) that coalesce to form large areas of caseation or they can break down into abscesses.

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15
Q

These two animals are highly susceptible to B. pseudomallei.

A
  1. Guinea pigs
  2. Rabbits
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16
Q

When B. pseudomallei is inoculated in male guinea pigs, what is the resulting clinical disease?

A

Develop Stratus reaction which produces a Purulent orchitis (an inflammation of the testicles that can involve pus or blood in the surrounding fluids).

17
Q

Give the diagnostic methods of B. pseudomallei.

A
  • Isolation and identification from blood and lesions
  • Hemagglutination test from serum
  • Fluorescent antiserum
  • Complement fixation test and agglutination test
18
Q

B. pseudomallei is resistant to what antibiotics?

A
  1. Cloxacillin
  2. Colistin
  3. Gentamicin
  4. Polymyxin B
19
Q

What are the preferred antibiotics against B. pseudomallei?

A
  1. Combination of trimethoprim and sulfamethoxazole,
  2. Novobiocin
  3. Tetracycline

BACTERIOLOGY & MYCOLOGY LECTURE (27 decks)

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