DNA extraction Flashcards
how do we collect the cheek cells
bite the insides of cheek, rinse water, spit
why is RNA used to transfer info from nucleus to cytosol
DNA cant leave nucleus for protection and security. if it goes outside, it will change easily and get destroyed
how to break cell
use detergent to dissolve membrane
use lipase to break down the lipids
protease
enzymes that break peptide bonds and does not come from E.Coli. In ecoli functions best at 37-38
Here at 50.
what is meat tenderizer
a powder or spice, an enzyme
that hydrolyzes structural proteins.
how to make DNA insolubale
salt
DNA has negative
salt is positive
DNA is now neutral
not soluble in water
how to percipate DNA
seperate it from other molecules, add cold alcohol
when you add it , DNA wil percipitate because it is less soluble in alcohol than in water.
when cold alcohol and water meet, DNA meets and comes together and is not dissolved at all. other stuff is dissolved.
