Diagnostics 4 - Histopathology and Cytopathology lab

Question 1

Differentiate histopathologists and cytopathologists

Answer 1

Histopathologists (tissue structure) - Biopsies, resection specimens, frozen sections, post mortems

Cytopathologists = smears and fine needle aspirates

Question 2

What are frozen sections fixed in?

Answer 2

Formalin - crosslinks proteins and slows decomposition

Then embedded in paraffin wax

Question 3

What stain is used for frozen sections

Answer 3

H + E (haematoxylin and eosin), ZN stain (for TB)

Question 4

When is cytopathology used?

Cytopathology is very quick

Answer 4

When looking at individual cells and not tissues

used for fine needle aspirations

used for cervical screening

Question 5

Which disease is the HIV/AIDS defining diease

Answer 5

Kaposis sarcoma - may be shown on a skin biopsy

Question 6

CD31 immunocytochemistry may show?

Answer 6

Vascular tumour infiltrating collagen bundles - endothelial cell tumour

CD31 marks vascular endothelium