D1.1

Question 1

Which of the following best describes DNA replication?

Answer 1

The formation of exact copies of DNA for reproduction and growth

Question 2

State the enzyme that is responsible for unwinding the DNA double helix during replication.

Answer 2

DNA helicase

Question 3

What does the semi-conservative nature of DNA replication ensure?

Answer 3

Accurate copying of base sequences

Question 4

Semi conservative definition

Answer 4

Daughter cell has one exact strand from parent and one newly formed strand

Question 5

Purines

Answer 5

Adenine, Guanine

Question 6

Pyrimidines

Answer 6

Cytosine, Thymine

Question 7

DNA primase:

Answer 7

lays down RNA primer so DNA polymerase III knows where to start adding nucleotides

Question 8

DNA polymerase III, polymerase I

Answer 8

adding nucleotides by complementary base pair,

• polymerase I removes RNA primer and adds nucleotide

Question 9

Ligase role:

Answer 9

connects okazaki fragments; creation of sugar and phosphate bond between nucleotides (phosphodiester bond)

Question 10

Errors in DNA are ___

Answer 10

mutations

Question 11

Proofreading to avoid mutations are done by _____.

Answer 11

polymerase III; replaces incorrect with correct nucleotide

Question 12

at the end of replication you should have ____

Answer 12

2 daughter strands.