Chapter 8 Flashcards
what is the central dogma
the overall flow of genetic information within the cell
3 steps of the overall flow of information (central dogma)
DNA –> RNA –> Protein
mutations can be caused by
base substitutions or frameshift mutations
genetics
the study of genes, how they carry information, how information is expressed, and ow genes are replicated
chromosome
structures containing DNA that physically carry hereditary information; the chromosome contain genes
Genes
segments of DNA that encode functional products, usually proteins
Genome
all the genetic information in a cell
genetic code
set of rules that determines how a nucleotide sequence is converted to an amino acid sequence of a protein
genotype
the genetic make up of an organism
genotype represents _______ expression
potential
phenotype represents ________ expression
actual
bacteria usually have a _______ circular chromosome made of ____ and associated _________
single, DNA, protein
the chromosome is _______ and ________ and attached at one or several points to the _________ ________
looped, folded, plasma membrane
how much volume does the chromosome take up
10%
why does a chromosome only take up 10% volume
decays the DNA is supercoiled
the entire genome does not consist of __________
back-to-back genes
short tandem repeats
repeating sequences of noncoding DNA
short tandem repeats can be used in
DNA fingerprinting
Genomics
the sequencing and molecular characterization of genomes
computers are used to search for opening reading frames
regions of DNA likely to encode a protein
vertical gene transfer
flow of genetic information from one generation to the next
DNA is _________ into mRNA and then __________ into protein
transcribed, translated
DNA is the blueprint for a cells _______
proteins
DNA is obtained
from another cell in the same generation
or
from a parent cell during cell division
DNA can be expressed within a cell or transferred to another cell through __________ and ___________
recombination and replication
expression
genetic information is used within a cell to produce the proteins needed for the cell to function
recombination
genetic information can be transferred horizontally between cells of the same generation
replication
genetic information can be transferred vertically to the next generation of cells
DNA backbone consists of
deoxyribose-phosphate
DNA strands are
antiparallel
in DNA order of nitrogen containing bases forms the
genetic instructions of the organism
DNA replication one strand serves as a template for _________ of a second strand
production
in DNA replication one parental double stranded DNA molecule is converted to
two identical offspring moleules
in DNA replication _______ and ________ relax the strand
topoisomerase, gyrase
in DNA replication _________ separated the strands
helicase
in DNA replication a __________ ____ is created
replication fork
in DNA replication DNA _________ adds _________ to the growing DNA strand
polymerase, nucleotides
DNA replication works in the __ to __ direction
5’, 3’
DNA replication adding nucleotides is initiated by
RNA primer
DNA replication leading strand is synthesized
continuously
DNA replication lagging strand is synthesized discontinuously creating a
Okazaki fragment
DNA replication DNA polymerase removes ___________; Okazaki fragments are joined by the DNA polymerase and _________
RNA primers, DNa ligase
Events at the DNA replication fork step 1:
enzyme unwind the parental _______ _____
double helix
Events at the DNA replication fork step 2:
proteins ______ the unwound parental DNA
stabilize
Events at the DNA replication fork step 3:
the leading strand is synthesized _________ by ___ ________
continuously, DNA polymerase
Events at the DNA replication fork step 4:
the lagging strand is synthesized ___________. ______, an RNA polymerase, synthesizes a short ____ ________ which is then extended by ____ ________
discontinuously, Primase, RNA primer, DNA polymerase
Events at the DNA replication fork step 5:
DNA polymerase digests ____ _______ and replaces it with ___
RNA primer, DNA
Events at the DNA replication fork step 6:
____ _______ joins the discontinuous fragments of the lagoon strand
DNA ligase
most bacterial DNA replication is
bidirectional
replication is highly accurate due to the proofreading capability of
DNA polymerase
DNA replication is the process of
copying DNA
rRNA
integral part of ribosomes
tRNA
transports amino acids during protein synthesis
mRNA
carriers coded information from DNA to ribosomes
Prokaryote Transcription begins when
RNA polymerase binds to the promoter sequence on DNA
prokaryote transcription proceeds in the ______ direction
5’–>3’
prokaryotes are both DNA strands or just one transcribed
only one
prokaryotes transcription stops when it reaches the
terminator sequence
transcription step 1:
RNA polymerase binds to the _____, and DNA unwinds at the beginning of the ______
promoter, gene
transcription step 2:
RNA is synthesized by complementary base paring of free ________ with the nucleotide bases on the template strand of DNA
nucelotides
transcription step 3:
the site of synthesis moves along ____; DNA that has been transcribed ______
DNA, rewinds
transcription step 4:
transcription reaches the ________
terminator
transcription step 5:
RNA and RNA polymerase are _____ and the DNA helix ______
released, reforms
3 phases of transcription
Initiation, elongation, termination
initiation step 1:
RNA polymerase binds to the DNA and recognizes a site called a ________ at the end of ___
promoter, 3’
initiation step 2:
when RNA polymerase finds a promoter it breaks the ________ _____ holding the DNA strands together at the site of the _________
hydrogen bonds, promoter
RNA polymerase does not bind to all promoters with
equal affinity
the difference in promoter strength is one way that cells can control
gene expression
the more strongly RNA polymerase binds to a particular promoter the more likely the gene is to be
transcribed
increase levels of transcription leads to increase levels of _________ leads to increased concentrations of that ___________
translation, polypeptide
elongation step 1:
one strand of DNA serves as a template strand. The RNA transcript is copied from this strand of the gene and therfor has an _____________ complementary to this strand
RNA sequence
elongation step 2:
RNA polymerase moves along the ___, opening up a bubble in the DNA
DNA
elongation step 3:
as it moves RNA polymerase adds bases to the __ end of the growing RNA transcript
3’
elongation step 4:
this continues until RNA polymerase reaches a site on the DNA called the ________
terminator
termination step 1:
at this side RNA polymerase and the newly synthesized RNA transcript are _______ from the DNA
released
termination can occur in 2 ways
self terminator and enzyme dependent termination
self termination:
the RNA sequence transcribed at the terminator causes the RNA to ________ ____ with it self forming a ____ ____ ______ which essentially pulls the RNA polymerase off the DNA
hydrogen bond, stem loop structure
enzyme dependent termination:
a termination _____ binds to the terminator and pushes RNA polymerase off the DNA
protein
codons
groups of three mRNA nucelotides that code for a particular amino acid
61 sense codons encode the
20 amino acids