Chapter 13 Flashcards
Given our knowledge of genome sizes in different organisms, would you predict that Homo sapiens or the two-toed salamander (Amphiuma means) has the larger genome?
you can not tell, you can not predict size from genomes
What is polyploidy?
possessing more than two complete sets of chromosomes, mainly in plants
Organisms that have been modified to contain DNA from other species are known as:
transgenic organisms
The type of genetic engineering that often involves isolating genes from one species and introducing them into another is called _____ technology.
recombinant DNA
Whole genome sequencing is often approached by a shotgun sequencing technique in which large genomes are:
randomly digested, short fragments are sequenced, and the overlapping sequences are assembled in order
In recombinant DNA experiments, ______ is used to cut pieces of DNA, and ______ joins these segments.
a restriction enzyme; DNA ligase
What sequences are longer repeated sequences or sequences obtained by automated sequencing
repeated sequencing
Restriction enzymes recognize certain DNA sequences and some of them will cut straight through, and others will leave an overhang at both ends of the cut.
True
The extrachromosomal DNA often found in bacteria is called a:
plasmid
What is the difference between PCR and Sanger sequencing with regard to the materials needed to perform these reactions?
Dideoxynucleotides are needed in Sanger sequencing but not in PCR.
In gel electrophoresis, DNA fragments migrate toward the negative pole of the electric field.
False, they move towards the positive end
A Southern blot is a technique that relies on hybridization of:
a nucleic acid probe to a complementary DNA.
Where is PCR used?
The lab
Are there more molecules of DNA in the 22 kb band or the 4 kb band on the gel?
Both bands have the same number
Which DNA band on the gel has a higher intensity of fluorescence, the 22 kb band or the 4 kb band?
22 kb
Where do restriction enzymes come from
bacteria
