Chapter 12 Flashcards
for a prokaryote with a single chromosome, what is the genome sequence?
a continuous string of base pairs
high-throughput sequencing
use miniaturisation techniques to sequence DNA
bioinformatics
analyse DNA sequences using complex mathematics and computer programs
functional genomics
use sequence information to identify the functions of various parts of genomes
open reading frames
sequences of DNA that have no stop codons, and can encode parts of proteins
give some examples of regulatory sequences
promoters and terminators
comparative genomics
comparison of a newly sequenced genome with sequences from other organisms
proteome
sum total of the proteins produced by an organism
why do proteins have unique combinations of electric charge and size?
due to their unique amino acid compositions
proteomics
identify and characterise all of the expressed proteins
metabolome
the complete set of small molecules present in a cell, tissue, or organism
primary metabolites
involved in normal processes
secondary metabolites
unique to particular organisms or groups of organisms
metabolomics
describe the metabolic profile of a tissue or organism under particular environmental conditions
how can molecules be identified?
through mass spectrometry and nuclear magnetic resonance spectroscopy
what are some notable features of bacterial and archaeal genomes?
small, compact, genes don’t contain introns, carry plasmids