Chapter 10: From DNA to Protein: Gene Expression Flashcards
Is the one gene-one protein hypothesis completely accurate?
no it is an oversimplification
Is the one gene-one polypeptide hypothesis complete accurate?
Whiel this is very useful, it is still a little simpler than real life (some genes do not code for polypeptides)
Molecular Biology
study of nucleic acids and proteins, often focuses on gene expression
True or false: genes are expressed as RNA
true
True or false: all RNA is translated into proteins
false
Transcription
the information in a DNA sequence (a gene) is copied into a complementary RNA sequence
Translation
the RNA sequence is used to create the amino acid of a polypeptide
Messenger RNA
the modified RNA that came from the complimentary DNA strand
Where does mRNA travel in eukaryotic cells?
from the nucleus to the cytoplasm
gets translated into a polypeptide
nucleotide sequence of it determines the sequence of the amino acids
Ribosomal RNA
rRNA
catalyzes peptide bond formation between amino acids to form a polypeptide
ribosome
protein synthesis factory
multiple proteins and rRNAs
Transfer RNA
tRNA
mediates between mRNA and protein
can bind a specific amino acid and recognize a specific sequence in mRNA
figures out which amino acid should be added to the growing polypeptide
What does DNA expression start with?
transcription to RNA
Transcription
tue formation of a specific RNA sequence from a specific DNA sequence
What factors are required for transcription?
A DNA template for complementary base pairing
the appropriate nucleoside triphosphates (ex ATP) to act as substrates
an RNA polymerase `
RNA polymerases
catalyze the synthesis of RNA from the DNA template
all have common structure
do not require primer
Are RNA polymerases processive?
Yes
a single enzyme-template binding event results in the polymerization of hundreds of RNA nucleotides
what are the three steps in transcription?
initiation
elongation
termination
Initiation (transcription)
needs a promoter
RNA polymerase binds to the promoter
Promoter
a special DNA sequence to which the RNA polymerase can bind to very tightly
tell RNA polymerase where to start transcription and which of the two strands to transcribe
orients the RNA polymerase
Tom Haverford
transcription initiation site
part of the promoter
where transcription begins
upstream
5’ on the non-template strand and 3’ on the template strand
Are all promoters identical?
no
Elongation (transcription)
the RNA polymerase unwinds the DNA and reads in the 3’ to 5’ direction (copying in the 5’ to 3’)
RNA polymerase uses the ribonucleoside triphosphates as substrates and catalyzes the formation of phosphodiester bonds
True or false: RNA polymerases and crew have more efficient proofreading mechanisms than DNA
false they are less efficient
True or False; RNA errors are more harmful than DNA ones
false
lots of RNA copies are made/they aren’t around for very long, less potential for harm in copying errors
Termination (transcription)
occurs at specific sequences
Coding reigons
sequences within a DNA molecule that are expressed as proteins
introns
intervening regions
interrupt the coding region
exons
expressed regions
transcribed regions
pre-mRNA
exons and introns (basically the whole section complementary to the DNA)
mature mRNA
just exons, no introns
Nucleic Acid Hybridization
DNA that you want analyzed denatured and hydrogen bonds broken to separate the pairs
probe incubated within the DNA if it has a base sequence complementary to the target DNA, double helix forms
prode
a single-stranded nucleic acid from another source
Do introns scramble the DNA sequence of a gene?
no they interrupt it
RNA splicing
removes the introns and splices the exons together
Consensus sequences
short stretches of DNA that appear with little variation in different genes that acts as borders between introns and exons
snRNOs
small nuclear ribonucleoprotein particles
surround the consensus sequences
has RNA with complementary sequences to the consensus sequences