Chap 4 Flashcards
comes from the Greek words for prenucleus.
Prokaryote
comes from the Greek words for true nucleus.
Eukaryote
One circular chromosome, not in a membrane
Prokaryote
No histones
Prokaryote
No organelles
Prokaryote
Bacteria: peptidoglycan cell walls
prokaryote
Archaea: pseudomurein cell walls
Prokaryote
Divides by binary fission
Prokaryote
Paired chromosomes, in nuclear membrane
Eukaryote
has Histones
Eukaryote
Has organelles
Eukaryote
Polysaccharide cell walls, when present
Eukaryote
Divides by mitosis
Eukaryote
What is the main feature that distinguishes prokaryotes from eukaryotes?
Prokaryotic cells have genetic material that is not contained in a nucleus
Average size of bacteria
0.2 to 2.0 μm diameter × 2 to 8 μm length
monomorphic bacteria
single shape
pleomorphic bacteria
many shapes
Bacillus
rod-shaped
coccus
spherical-shaped
Types of spiral bacteria
Vibrio
Spirillum
Spirochete
Shapes of bacterial cells
1.Bacillus (rod-shaped)
2.Coccus (spherical-shaped)
3.Spiral
4.Star-shaped
5.Rectangular
See fig. 4.4 Draw types of spiral bacteria
diplococci, diplobacilli
pairs di-
di-
pairs
staphylo-
clusters staphylococci
Strepto-
Chains
tetrads
groups of 4
sarcinae
cubelike groups of 8
Bacillus scientific name and shape
scientific name: Bacillus
Shape:bacillus
vibrio
one curve
Spirilum shape
more curved than vibrio, thicker than spirochete
Spirochete
more slender than spirilum
How can you identify streptococci with a microscope?
it would look like a chain of spheres under the microscope
Glycocalyx is external to
External to the cell wall
Consistency of glycocalyx
viscous and gelatinous
Glycocalyx is made of
Made of polysaccharide and/or polypeptide
Two types of glycocalyx
1.Capsule
2. Slime layer
Capsule glycocalyx
Neatly organized and firmly attached
Slime layer glycocalyx
unorganized and loose
Glycocalyx contributes to
virulence
the relative capacity of a microbe to cause damage in a susceptible host
virulence
virulence
the relative capacity of a microbe to cause damage in a susceptible host
Cause of Pneumococcal Pneumonia
Streptococcus pneumoniae
Filamentous appendages external of the cell
Flagella
Function of flagella
Propel bacteria
Flagella is made of
protein flagellin
Filament of flagella
Outermost region
Hook of flagella
attaches to the filament
Basal body of flagella:
consists of rod and pairs of rings; anchors flagellum to the cell wall and membrane
Parts of flagella
- Filament
- Hook
- Basal body
Monotrichous
cells with a single flagellum
in monotrichous cells, what happens when the flagellum rotates counterclockwise?
The bacterium runs in a single direction
in monotrichous cells, what happens when the flagellum rotates clockwise?
The bacterium tumbles and changes direction
amphitrichous bacteria
have a flagellum at either end of the cell
lophotrichous
bacteria with a tuft of flagella at one end. Multiple tails at one end
in lophotrichous cells, what happens when the flagella rotate counterclockwise?
clockwise?
counterclockwise: the bacteria runs
clockwise: the bacteria tumbles
Peritrichous bacteria
have flagella covering the surface of the cell
When does Peritrichous bacteria run?
when all flagella rotate counterclockwise and become bundled.
When does Peritrichous bacteria tumble?
When flagella rotate clockwise and separate
Importance of flagella during taxis
Flagella allow bacteria to move toward or away from stimuli
Why do flagella rotate?
to “run” or “tumble”
Flagella proteins are what kind of antigens ?
Flagella proteins are H antigens
serovars
different strains of bacteria (called serovars, for serological variants)
Helps distinguish among serovars
flagella proteins
motility
The ability of an organism to move by itself
Why is motility of cells important?
Lets the cell move towards a desired environment or to flee from a harmful environment
Has axial filaments
spirochetes
Taxis
movement toward or away from a stimulus
phototaxis
light stimulus
chemotaxis
chemical stimulus
When do tumbles become less frequent?
positive taxis
positive taxis
move towards the stimulus
When do tumbles become more frequent?
When the cell moves away from the stimulus
Archaella
Another motility structure. Found on archaea. Are thinner and more flexible than flagella. Made of different proteins than flagella.
Archaella are made of
glycoproteins archaellins
Archaella are anchored to
the cell
motion of archaella
rotate like flagella
Axial filaments are also called
endoflagella
Axial filaments are found in
spirochetes
Where are axial filaments anchored?
at one end of the cell
What kind of movement do axial filaments create?
Rotation causes cell to move like a corkscrew
Group of bacteria that are spiral shaped. Move in corkscrew-like fashion
spirochetes
Use flagella that do not protrude from the cell wall like typical flagella
spirochetes
Lyme disease is caused by a
spirochete
Borrelia burgdorferi
spirochete that causes lyme disease
treponema pallidum
causes syphilis, spirochete
The axial filament is made of a bundle of flagella called
endoflagella
How does the endoflagella cause the spirochete to move?
rotation of the endoflagella around the bacterium causes the spirochete to corkscrew through the medium. Lets it move through mucus easily
Fimbriae
Hairlike appendages that allow for attachment
Pili involved in
Involved in motility (gliding and twitching motility)
Conjugation pili involved in
DNA transfer from one cell to another
Why are bacterial capsules medically important
Capsules often protect pathogenic bacteria from phagocytosis by the cells of the host.
How do bacteria move?
Conjugation, motility, taxis
conjugation
the process by which one bacterium transfers genetic material to another through direct contact
what does the cell wall prevent?
Prevents osmotic lysis and protects the cell membrane
In bacteria, the cell wall is made of
peptidoglycan
the cell wall contributes to
pathogenicity
Pathogenicity
the ability of an organism to cause disease
Peptidoglycan is made of
Polymer of a repeating disaccharide in rows:
N-acetylglucosamine (NAG)
N-acetylmuramic acid (NAM)
Row in peptidoglycan are linked by
polypeptides
Characteristics of Gram-positive Cell walls
1.Thick peptidoglycan
2.Teichoic acids
Peptidoglycan
A biopolymer consisting of amino acids and carbohydrates, forming the cell wall of most bacteria.
Teichoic acids
Bacterial polysaccharide. They are the major components of the cell walls and membranes of many bacteria.
Characteristics of gram-negative cell walls
1.Thin peptidoglycan
2.Outer membrane
3.Periplasmic space
Function of teichoic acids
Carry a negative charge
Regulate movement of cations
Lipoteichoic acid
links cell wall to plasma membrane
Wall teichoic acid
links the peptidoglycan