Chap 11 Flashcards
The Prokaryotes: Domains Bacteria and Archaea
Prokaryotes in Bergey’s manual are grouped into two domains
Archaea & Bacteria
Domain of prokaryotic cells lacking in peptidoglycan
archaea
bacteria are also commonly distinguished by gram staining
gram positive or gram negative
proteobacteria characteristics
1.Gram-negative,
2.chemoheterotrophic bacteria that posses a
3.signature rRNA sequence
proteobacteria are thought to have arised from
a common photosynthetic ancestor
Largest taxonomic group of bacteria
proteobacteria
Few proteobacteria are
photosynthetic, other capacities have replaced this characteristic
The phylogenetic relationship between proteobacteria is based upon
rRNA studies
The name proteobacteria comes from
Greek god Proteus, who could take many shapes
Proteobacteria are designated into five classes
- alphaproteobacteria
- beta
- gamma
- delta
- epsilon
Alphaproteobacteria capable of growth with very low levels of ______
nutrients
prosthecae
unusual stalks or buds that grow out of alphaproteobacteria
prostheca
a stalk or bud protruding from a prokaryotic cell
Include agriculturally important bacteria capable of nitrogen fixation
alphaproteobacteria
Pelagibacter Ubique
most abundant living organism in the oceans on the basis of weight.
Thought to have an important role in the earth’s carbon cycle
P. ubique
P. Ubique has a small size and small genome, which allows it to
survive in low-nutrient environment
size of P. ubique
0.3 micrometers in diameter
Which bacteria have the smallest genomes?
bacteria in symbiotic relationships because they have lower metabolic requirements
What has a smaller genome than P. ubique?
several mycoplasmas
has only 1354 genes
P. Ubique
first member of SAR 11 group to be successfully cultivated
P. ubique
Azospirilum
soil bacterium; grows closely associated to roots, especially in tropical grasses. Uses nutrients excreted from the plants to fix nitrogen from atmosphere.
azo-
nitrogen fixing
azo- comes from
without life, nitrogen associated with no life
Acetobacteraceae & Gluconobacter
industrially important 1. aerobic organisms that
2.convert ethanol into acetic acid (vinegar).
Granulibacter
emerging; found in patients with chronic granulomatous disease.
In the first edition of Bergey’s Manual, the genera Rickettsia, Coxiella, and Chlamydia were grouped closely because
all obligate intracellular parasites—that is, they reproduce only within a mammalian cell
In the second edition they are now widely separated.
Rickettsias, chlamydias, and viruses
Contain a single type of nucleic acid, either DNA or RNA
viruses
Contain a protein coat (sometimes itself enclosed by an envelope of lipids, proteins, and carbohydrates) that surrounds the nucleic acid.
viruses
Multiply inside living cells by using the synthesizing machinery of the cell
viruses
Cause the synthesis of specialized structures that can transfer the viral nucleic acid to other cells.
viruses
Rickettsias gram negative or gram positive?
gram-negative
One distinguishing feature of most rickettsias
transmitted to humans by insect and tick bites
Rickettsia enter their host cell by
Inducing phagocytosis. They quickly enter the cytoplasm of the cell and begin reproducing by binary fission
Rickettsias can usually be cultivated artificially in
cell culture or chick embryos
are responsible for a number of diseases known as the spotted fever group.
Rickettsias
epidemic typhus, caused by
Rickettsia prowazekii
Epidemic typhus is transmitted by
transmitted by lice
endemic murine typhus, caused by
R. typhi
endemic murine typhus, transmitted by
rat fleas
Rocky Mountain spotted fever, caused by
R. rickettsii, transmitted by ticks
In humans, rickettsial infections damage
The permeability of blood capillaries, which results in a characteristic spotted rash.
Ehrlichia
1.gram-negative, 2.rickettsia-like bacteria that
3. live obligately within white blood cells
Ehrlichia species are transmitted by
ticks to humans
cause ehrlichiosis, a sometimes fatal disease
Ehrlichia
Caulobacter and Hyphomicrobium found in
low-nutrient aquatic environments, Lakes
Have stalks that anchor the organisms to surfaces
Caulobacter and Hyphomicrobium
Waht is the function of the stalk in Caulobacter and Hyphomicrobium?
Increases nutrient uptake
How does the stalk increase nutrient uptake?
-exposed to changing flow of water, increases surface area,
-can use host excretions as nutrients
When does the stalk grow?
When the nutrient concentration is exceptionally low, to pick up more food
Budding bacteria don’t divide by
binary fission into two identical cells
What happens during the budding process?
The parent cell retains its identity while the bud increases in size until it separates as a complete new cell.
Hyphomicrobium found in
in low-nutrient aquatic environments
Caulobacter and Hyphomicrobium reproduce by
budding rather than binary fission
Both Caulobacter and Hyphomicrobium produce prominent
prosthecae
Rhizobium, Bradyrhizobium infect
roots of leguminous plants, such as beans, peas, or clover
common name of Rhizobium, Bradyrhizobium
rhizobia
presence of rhizobia in the roots leads to
formulation of nodules;
Rhizobia and plant form a symbiotic relationship that results in
fixation of nitrogen from the air so it can be used by the plant
Agrobacterium can invade
plants
can invade plants but these bacteria do not induce root nodules or fix nitrogen
Agrobacterium
Agrobacterium tumefaciens causes a disease called
crown gall
Plant crown
Area of plant where roots and stem merge
How does crown gall occur?
A. tumefaciens inserts a plasmid containing bacterial genetic information into the plant’s chromosomal DNA
Plasmids
most common vector that scientists use to carry new genes into a plant cell
Why are plasmids the most common vector that scientists use to carry new genes into a plant cell?
Thick wall of plants is hard to penetrate
Genus __________ has several members that are human pathogens
Bartonella
Bartonella henselae
1.gram-negative bacillus that
2.causes cat-scratch disease
Brucella
obligate parasites of mammals, cause of brucellosis
small nonmotile coccobacilli, cause brucellosis
Brucella
Brucella can survive
phagocytosis
Nitrobacter and Nitrosomonas are important to
environment and agriculture; nitrifying bacteria
Obligate parasite of mammals; survives phagocytosis
Causes brucellosis
Brucella
Nitrobacter and Nitrosomonas are chemoautotrophic, meaning
use inorganic chemicals as energy source; CO2 as carbon source
Nitrosomonas equation
NH4 —> NO2-
Nitrobacter equation
NO2- —> NO3-
Wolbachia
Endosymbiont of insects
Affects reproduction of insects
The energy sources of the genera Nitrobacter and Nitrosomonas (the latter is a member of the betaproteobacteria) are
reduced nitrogenous compounds
Nitrate is important to agriculture;
it’s a nitrogen form that’s highly mobile in soil and therefore likely to be encountered and used by plants.
most common infectious bacterial genus in the world
Wolbachia
Wolbachia live only inside the cells of
their hosts
hosts of wolbachia are usually
Insects
escapes detection by the usual culture methods.
Wolbachia
interferes with reproduction and egg development in infected insects.
Wolbachia
Wolbachia-infected male Aedes aegypti mosquitoes are being released in several places including Brazil, Florida, California, and southeast Asia to
prevent the spread of Zika, chikungunya, and dengue viruses
The Betaproteobacteria
- Acidithiobacillus
- Spirillum
- Sphaerotilus
Found in freshwater
Move via flagella
Spirirlum
Acidithiobacillus
1.Chemoautotrophic;
2.oxidize sulfur to sulfates:
H2S —> SO4 2-
Sphaerotilus function
Form sheaths to aid in protection and nutrient gathering
The betaproteobacteria often use nutrient substances that diffuse away from
areas of anaerobic decomposition of organic matter, such as hydrogen gas, ammonia, and methane. Several important pathogenic bacteria are found in this group.
sulfur-oxidizing bacteria are important in the sulfur cycle
Acidithiobacillus
chemoautotrophic bacteria capable of obtaining energy by oxidizing the reduced forms of sulfur, such as hydrogen sulfide (H2S), or elemental sulfur (S0), into sulfates (SO42−).
Acidithiobacillus
________ bacteria are motile by conventional polar flagella
Spirillum
Relatively large, gram-negative, aerobic bacteria.
Spirilla
Sphaerotilus gram negative or gram positive?
- gram-negative
Sheaths aid in
protection and nutrient accumulation
Sphaerotilus natans contributes to
bulking in sewage
can eventually swim free of the sheath.
Sphaerotilus
Burkholderia motile by
single polar flagellum or tuft of flagella
Burkholderia cepacia aerobic vs anaerobic
aerobic,
nutritional spectrum of Burkholderia cepacia
capable of degrading more than 100 different organic molecules.
The large nutritional spectrum of burkholderia is a problem because
bacteria can grow in diinfectant solutions. Contamination of equipment and drugs in hospitals
Why is burkholderia a problem for people with genetic lung disease cystic fibrosis,?
it metabolizes accumulated respiratory secretions.
Burkholderia pseudomallei found in
Moist soils in southeast asia and northern australia
causes melioidosis
Burkholderia pseudomallei
Bordetella pertussis characteristics
nonmotile, aerobic, gram-negative
cause of pertussis or whooping cough
Bordetella pertussis
Neisseria characteristics
aerobic, gram-negative cocci
Neisseria usually inhabit
mucous membranes of mammals
Neisseria gonorrhoeae
Causative agent of gonorrhoea
agent of meningococcal meningitis
N. meningitidi
uses fimbriae and an outer membrane protein called Opa to attach to host cells. After the (red) bacterium attaches, the (green) host cell membrane surrounds it
Neisseria gonorrhoeae.
How do fimbriae contribute to pathogenicity?
helping bacteria adhere to surfaces and invade host cells
As they grow, Zoogloea bacteria form
fluffy, slimy masses that are essential to the proper operation of aerobic sewage treatment
largest subgroup of the proteobacteria and include a great variety of physiological types
Gammaproteobacteria
Thiomargarita namibiensis,
the largest known bacterium but also exhibits several unusual characteristics
Thiotrichales Beggiatoa
nutritionally distinctive
pathogen causing tularemia
Francisella tularensis
Beggiatoa species grows in
unusual genus that grows the only in aquatic sediments between aerobic and anaerobic layers
Morphologically, the genus resembles certain filamentous cyanobacteria, but it is not photosynthetic
Beggiatoa
Its motility is enabled by the production of slime
Beggiatoa
How does slime aid motility?
attaches to the surface on which movement occurs and provides lubrication, allowing the organism to glide
Francisella
F. tularensis:
causes tularemia
Pseudomonadales
Pseudomonas are what kind of pathogens?
Opportunistic pathogens; nosocomial infections
Pseudomonadales
Pseudomonas
metabolism
Metabolically diverse
Motility of pseudomonas
Polar flagella; common in soil