Ch. 8 Gene Expression Flashcards
process of using instructions carried by a gene to build a particular protein
protein synthesis
coping of DNA into RNA. happens inside nucleus
transcription
enzyme that binds to promoter to begin transcription. unzips DNA to make RNA template. creates complementary messenger RNA (mRNA)
RNA polymerase
mRNA is blueprint for protein. happens in cytoplasm
translation
made of rRNA. large and small subunit come together and mRNA is threaded between them
ribosome
carries specific amino acid on the anticodon
transfer RNA (tRNA)
3 nucleotides long and encode a particular amino acid on the mRNA
codon
3 nucleotides long. bind to codon if match. complementary to codon. adds amino acid to growing polypeptide
anticodon
codon that does not code for an amino acid. growing protein is then released and folds upon itself and moves to required location within cell
stop codon
determines which mRNA codons code for which amino acids
genetic code
AUG
start codon
UAA, UAG, UGA
stop codon
changes to DNA sequence
mutations
mutation has no effect on protein
neutral mutation
insertion or deletion of nucleotides shifts the reading frame
frameshift mutation
triplet groupings of nucleotides
reading frame
turning a gene on or off in response to cells needs
regulating gene expression
undifferentiated and do not perform a specific function
stem cells
using stem cells to produce healthy tissues to replace damaged tissues
therapeutic cloning
stem cells are able to become any other cell
totipotent
determining nucleotide base sequence for the entire human genome and the location of each of the genes
human genome project
important genes are often common in many organisms other than humans
model organism
replacing defective genes with functional genes
gene therapy
supplying embryo with normal versions of defective gene to be replicated. making most cells carry the functioning gene
germ-line gene therapy