Ch 2 - Microscopy Flashcards

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1
Q

Microscopy that allows for the visualization of live samples

A

Phase-contrast

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2
Q

microscopy image is dark cells on a light background

A

phase-contrast

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3
Q

improves contrast of a sample without the use of dye

A

phase-contrast

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4
Q

microscopy image appears light on a dark background

A

dark-field microscopy

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5
Q

Light reaching the lens has been scattered by specimen

A

dark-field microscopy

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6
Q

stylus measures weak repulsive forces between it and the specimen

A

atomic force microscopy

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7
Q

Uses a computerized microscope coupled with a laser source to generate a three-dimensional image

A

Confocal Scanning Laser Microscopy

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8
Q

Computer can focus the laser on single layers of the specimen

A

Confocal Scanning Laser Microscopy

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9
Q

Specimen is coated with a thin film of heavy metal

A

scanning electron microscope

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10
Q

very large specimens can be observed

A

scanning electron microscope

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11
Q

Prokaryotic/eukaryotic cells generally have a single, circular DNA molecule called a chromosome

A

prokaryotic

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12
Q

Prokaryotic/ Eukaryotic DNA is linear

A

eukaryotic

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13
Q

Typically two copies of each chromosome: prokaryotic/eukaryotic

A

eukaryotic

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14
Q

may have small amounts of extra-chromosomal DNA called plasmids : prokaryotic/eukaryotic

A

prokaryotic

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15
Q

During cell division, nucleus divides by mitosis

: prokaryotic/eukaryotic

A

eukaryotic

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16
Q

Evolutionary relationships between organisms

A

phylogeny

17
Q

Obtain their energy from the oxidation of organic molecules

A

chemoorganotrophs

18
Q

Obtain their energy from the oxidation of inorganic molecules

A

chemolithotrophs

19
Q

use oxygen to obtain energy

A

aerobes

20
Q

obtain energy in the absence of oxygen

A

anaerobes

21
Q

Contain pigments that allow them to use light as an energy source

A

phototrophs

22
Q

this photosynthesis produces oxygen

A

oxygenic

23
Q

this photosynthesis does not produce oxygen

A

anoxygenic

24
Q

Use carbon dioxide as their carbon source

A

autotrophs

25
Q

Require one or more organic molecules for their carbon source

A

heterotrophs

26
Q

All known pathogenic prokaryotes are

A

bacteria

27
Q

______make up the largest phylum of Bacteria

A

proteobacteria

28
Q

are proteobacteria gram (-) or Gram (+)?

A

Gram (-)

29
Q

are cyanobacteria gram (-) or Gram (+)?

A

Gram (+)

30
Q

what are the two phyla in the Archae domain?

A

Euryarchaeota , Crenarchaeota

31
Q

degrade organic matter anaerobically, produce methane

A

methanogens

32
Q

require high salt concentrations for metabolism and reproduction

A

extreme halophiles

33
Q

grow in moderately high temperatures and low-pH environments

A

thermoacidophiles

34
Q

Vast majority of cultured Crenarchaeota

A

hyperthermophiles

35
Q

algae, fungi, protozoa, and slime molds: eukaryotic or prokaryotic

A

eukaryotic

36
Q

do algae and fungi have cell walls?

A

yes

37
Q

do protozoa and slime molds have cell walls?

A

no

38
Q

microbes with mutualistic relationship between two groups of protists

A

lichens