Ch 2 - Microscopy Flashcards
Microscopy that allows for the visualization of live samples
Phase-contrast
microscopy image is dark cells on a light background
phase-contrast
improves contrast of a sample without the use of dye
phase-contrast
microscopy image appears light on a dark background
dark-field microscopy
Light reaching the lens has been scattered by specimen
dark-field microscopy
stylus measures weak repulsive forces between it and the specimen
atomic force microscopy
Uses a computerized microscope coupled with a laser source to generate a three-dimensional image
Confocal Scanning Laser Microscopy
Computer can focus the laser on single layers of the specimen
Confocal Scanning Laser Microscopy
Specimen is coated with a thin film of heavy metal
scanning electron microscope
very large specimens can be observed
scanning electron microscope
Prokaryotic/eukaryotic cells generally have a single, circular DNA molecule called a chromosome
prokaryotic
Prokaryotic/ Eukaryotic DNA is linear
eukaryotic
Typically two copies of each chromosome: prokaryotic/eukaryotic
eukaryotic
may have small amounts of extra-chromosomal DNA called plasmids : prokaryotic/eukaryotic
prokaryotic
During cell division, nucleus divides by mitosis
: prokaryotic/eukaryotic
eukaryotic