Cell theory (CH 4.1) Flashcards
Who was the first person to observe cells?
- Robert Hooke in 1655
- Named shapes he saw in cork Cellulae (small rooms)
Who was the first person to observe LIVING cells?
- Anton Van Leeuwnehoek
- Termed “animacules”
What are 3 principles of Cell Theory?
- All organisms are composed of one or cells &; life processes of metabolism &’ heredity occur within these cells
- Cells are the smallest living things= basic units of organization of all organisms
- Cells come from the division of previous cells
Why are cells so small?
-Reasons related to diffusion of substances in &; out of them
What variables affect the rate of diffusion?
- Surface area
- Temperature
- Concentration gradient of diffusion subject
- Distance over where diffusion must occur
What is the relationship between the size of the cell &; diffusion?
- Directly proportional
- The bigger the cell, the longer the length of time for diffusion from outside of cell membrane to inside of cell
- Larger cells need to synthesize more macromolecules= higher energy needed= higher waste produced
What does the rate depend on between metabolic waste being removed &; transport through cell membrane?
-Distance of cell membrane &; the area of cell membrane available
What is Surface-Area to Volume Ratio?
- As cell size increases, it’s volume increases more rapidly than the surface area
- For spherical cells, surface area= Radius^2 &; volume= Radius ^3
Why are smaller cells better than bigger cells?
-Have more surface area per unit of volume= control of contents over contents is more effective
What is Resolution?
-Minimum distance between two points can be apart &; still be distinguished as two separate points
How do Modern Light Microscrope operate?
- Operate w/ visible light
- Use 2 magnifying glasses to achieve high magnification &; clarity
- 1st lense= focuses image of the 2nd lens &; magnifies it &; focuses on the back of the eye
What causes images from a microscope to overlap?
- Objects being closer than a few hundred nanometers
- Light beams reflecting off the object causes the image to overlap each other
How do you reduce overlapping of images for a microscope?
-Using electron beams because they have a shorter wavelength= has 1000x more power than a light microscope
What are the 2 kinds of electrons microscopes?
- Transmission electron microscope
- Scanning Electron Microscrope
What is Immunohistochemistry?
- Strains that bind to particular types of molecule to increase constrast between different cellular components
- Using antibodies generated in animals