Cell Cycle and Genetic Engineering Flashcards
Cell Continuity
The way in which cells arise from cells of the same type and organisms from organisms of the same type
Cell Cycle
Events in the life of a cell
Interphase
Cell not dividing
Organelles replicate
Energy builds up
Prophase
- Chromatin contracts
- Chromosome forms and duplicate
- 2 sisters chromatids
- Spindle fibres forming
- Nuclear membrane breaks down
Metaphase
- Spindle fibres completely formed
- Sister chromatically are aligned along fibres
- Chromatids are attached to spindle fibres by centromere
Anaphase
- Spindle contracts
- Sister chromatids pull apart to opposite poles
- Chromatids become chromosomes
Telophase
- Nuclear membrane forms
- Spindle breaks down
- Chromosomes elongate and disappear
- Cytoplasm divides in two halves
How does Cytokinesis happen in Animal Cells
Cleavage furrow forms
What happens after Telophase in Plant Cells
Cell plate forms
Functions of Mitosis
- Reproduction in unicellular organisms
* Growth and repair in multicellular organisms
Cancer
A group of disorders caused by the uncontrollable rate of mitosis
Causes of Cancer
Oncogenes
Asbestos fibres
Cigarette smoke
Mitosis
A type of nuclear division in which the NUMBER of chromosomes of the daughter is the same as the parent nucleus
Meiosis
A type of nuclear division in which the daughter nuclei contains half the number of chromosomes as the parent nucleus
Stages in Genetic Engineering
Isolation Cutting Ligation Transformation Expression
Isolation
Plasmid from bacterial cell
Cells broken open
Genetic probe reveals position of gene of interest
Cutting
Restriction enzyme cut plasmid
Ligation
Rejoining cut fragments of DNA using DNA ligase
Transformation
Bacteria induced to take up recombinant plasmid
Expression
Getting organism with recombinant DNA to produce the desired product which is extracted
Genetic Engineering in Plants
Weed killer resistant crops
Genetic Engineering in Animals
Goats produce a protein to treat cystic fibrosis
Genetic Engineering in Micro-organisms
Bacteria make insulin