Biotech Principles & Processes

Question 1

EFB stands for….

Answer 1

European federation of biotechnology

Question 2

Two core techniques that enabled birth of modern biotechnology are

Answer 2

Genetic engineering

Bioprocess engineering

Question 3

Assertion:Modern rDNA technology is better than traditional hybridisation technology

Answer 3

Reason: It allows us to isolate and introduce only one or a set of desirable genes without introducing undesirable genes into the target organism.

Question 4

Stanley Cohen and Herbert Boyer accomplished constructing first artificial rDNA in

Answer 4

1972

Question 5

The ability to multiply copies of antibiotic resistance gene in
E. coli was called……..

Answer 5

cloning

Question 6

Three basic steps in genetically modifying an organism —

Answer 6

(i) identification of DNA with desirable genes;
(ii) introduction of the identified DNA into the host;
(iii) maintenance of introduced DNA in the host and transfer of the DNA to its progeny.

Question 7

In the year ……… the two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated

Answer 7

1963
One of these added methyl groups to DNA, while the other cut DNA. The later was called restriction endonuclease.

Question 8

Hind II always cut DNA molecules at a particular point by recognising a specific sequence of …………

Answer 8

six base pairs

Question 9

Today we know more than ………… restriction enzymes that have been isolated from over……….strains of bacteria

Answer 9

900

230

Question 10

EcoRI comes from……

Answer 10

Escherichia coli RY 13

Question 11

Restriction endonuclease cut each of the two strands of the double helix at specific points in their………

Answer 11

sugar-phosphate backbones

Question 12

Palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept the same or different?

Answer 12

same

Question 13

Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands
True or false

Answer 13

True

Question 14

Assertion:DNA Molecules can be separated by forcing them to move towards the anode under an electric field through a medium/matrix

Answer 14

Reason: DNA fragments are negatively charged molecules

Question 15

…………. is a natural polymer extracted from sea………

Answer 15

agarose

weeds

Question 16

For separating DNA fragments the sieving effect is provided by the……….

Answer 16

agarose gel

Question 17

Some plasmids may have only one or two copies per cell whereas others may have……….copies per cell

Answer 17

15-100

Question 18

Origin of replication sequence is not responsible for controlling the copy number of the linked DNA
True or false

Answer 18

False
This sequence is also responsible for controlling the copy number of the linked DNA

Question 19

presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria……

Answer 19

does not have an insert.

Question 20

Agrobacterium tumifaciens, a pathogen of several………plants is able to deliver a piece of DNA known as…….

Answer 20

Dicot t-dna

Question 21

Assertion: DNA cannot pass through cell membranes.

Answer 21

Reason: It is a hydrophilic molecule

Question 22

Heat shock for making cells take up DNA………*C

Answer 22

43 degree Celsius

Question 23

Cells are bombarded with high velocity micro-particles of…………. in a method known as biolistics or……

Answer 23

gold or tungsten coated with DNA

gene gun