Biomedical Laboratory Techniques (5/14) Flashcards
1
Q
PCR requirements
A
DNA polymerase
deoxynucleotides
reaction budder
primer specific to target sequence
2
Q
Steps of PCR
A
denature
annealing
extension- Taq polymerase
3
Q
Sanger sequencing requirements
A
ddNTPs, DNA polymerase, dNTPs, fluorescent markers, gel electrophoresis apparatus