Analytic techniques (5/16)

Question 1

Protein purification

Answer 1

extraction
separating
isolating protein of interest

Question 2

Extraction

Answer 2

must lysed proteins to let lose organelles

Question 3

Centrigugation

Answer 3

separates particles in solution by mass, shape, and density

Question 4

The bottom of the tube after centrifuging

Answer 4

pellet

Question 5

The top of the tube after centrifuging

Answer 5

supernatant

Question 6

Solubility

Answer 6

adding salt increases

too much added= salting add and solubility decreases

Question 7

Chromatography

Answer 7

used to isolate protein of interest

Question 8

Electrophoresis

Answer 8

separates molecules based on their migration in an electric field
anode (+)
cathode (-)

Question 9

Electrophoresis (SDS-page) protein migration distance depends on

Answer 9

charge
shape and size
-usually a basic pH so most proteins have a negative charge
-SDS makes charge state of proteins negligible (disrupts non-covalent interactions) to disrupt secondary
-other things added to disrupt tert and quart

Question 10

Isoelectric focusing

Answer 10

separates proteins with different charge states

separates proteins based on their relative number of acidic and basic residues

Question 11

2D gel electrophoresis

Answer 11

separate further by mass

Question 12

Immunoassays

Answer 12

use antibodies for specifics 
EX)
western blotting
radioimmunoassay 
ELISA

Question 13

Spectroscopy

Answer 13

can quantify the amount of protein in a sample based on its absorption of light at a wavelength
high absorbance= low transmittance of light

Question 14

Beer Lambert Law

Answer 14

A=Ecb=-Ln(I/I_0)