bacteria (gram) Flashcards
Define pathogen
Organisms that causes of is capable of causing disease
Define commensal
Organism which colonises the hose but causes no diseases in normal circumstances (staph aureus)
Define opportunistic pathogen
Microbe that only. causes disease if the host defences are compromised
Define virulence/pathogenicity
What is the resolving eye of the naked eye
100um
What is the resolving power of a light microscope?
0.2um
what is bacterial nomenclatus
Genus then species
what colour does gram negative stain
pink
What colour does gram-positive stain
Purple
where is DNA transcribed in a bacterium
Cytoplasm
what are the features of a gram positive cells
capsule
peptidoglycan thick
Lipoteichoic acid
What are the features of gram negative cells?
Capsules
peptidoglycan thin
LPS (ENDOTOXIN)
periplasmic space
Describe bacterial environments
can be stored at -80-+80
ph 4-9
can survive without water
some can survive UV
Some can survive radiation
What is the doubling time for E.coli
20-30 mins
what is the doubling time for S.aureus
20-30mins
What is the doubling time for mycobacterium, tuberculosis
24 hours
What is the doubling time for candida albicans
30 mins
What is the doubling time for Mycobacterium leprae
2 weeks
what are the 2 main toxins from bacterium
Endotoxin and exotoxin
endotoxin is LPS (gram neg)
What type of toxin is tetanus
Exotoxin
What cells produce exotoxin
Gram-positive and negative
What cells produce endotoxin
Gram negative LPS
How can bacteria transfer genes
Transformation via plasmid
transduction via phage
conjugation via pilli
what are the obligate intraceuular bacteria
Rickettsia
Chlamydia
Coxiella
What are the 3 classed of spirochaeted
Leptospira
TREPONEMA
BORRELIA
What are tej important GRAM NEGATIVE aerobic and anaerobic bacterium
Aerobic -NEISSERIA
N. meningitidis
N. gonorrhoeae
etc
Anaerobic - VEILLONELLA
What are the important GRAM POSITIVE aerobic and anaerobic bacterium
Aerobic - STAPHYLOCOCCUS
S. aureus
S. epidermidis
STREPTOCOCCUS
Anaerobic - PEPTOSTREPTOCOCCUS
What are the important streptococcus?
BETA-HAEMOLYTIC
S. pyogenes (A)*
S. agalactiae (B)*
etc
ALPHA-HAEMOLYTIC
S. pneumoniae
S. oralis
S. milleri
S. sanguis
etc
NON-HAEMOLYTIC
S. bovis
ENTEROCOCCUS
E. faecalis (D)*
what stain with the ziehl Neelsen stain-positive
Myobacteria
What are the GRAM POSITIVE rods? aerobic and anaerobic
ANAEROBIC - CLOSTRIDIUM
C. perfringens
C. tetani
C. botulinum
C. difficile
etc
PROPIONIBACTERIUM
P. acnes
AEROBIC -CORYNEBACTERIUM
C. diphtheriae
etc
LISTERIA
L. monocytogenes
etc
BACILLUS
B. anthracis
B. cereus
etc