Antibiotics Flashcards
What is an antibiotic?
Antibiotics are molecules that work by binding a target site on a bacteria
what class of antibiotic is penicillin
B lactam
List some B lactam antibiotics
penicillin derivatives
cephalosporins and cephamycins
monobactams
carbapenems and carbacephems
what does the antibiotic polymyxin work on
Cell Membrane
Treats gram negative infections, last line of antibiotics, narrow spec
What are the classes of antibiotics that work on 30s
Tetracyclines
Aminoglycosides
What are the classes of antibiotics that work on 50s
Macrolides
Clindamycin
Linezolid
Chloramphenacol
Streptogramins
what are the classes of antibiotics that work on RNA polymerase
Rifampin
What are the classes of antibiotics that work on DNA gyrase
Quinolones
What are the classes of antibiotics that work on folalte synthesis
Sulfonamides
Trimethoprin
What type of antibiotics are Vancomycin +
Teicoplanin
Glycopeptides
Treat beta lactam resistant gram +ve bacteria
Describe how Beta lactam antibiotics
work
- disrupt peptidoglycan production
- bind covalently and irreversibly to Penicillin Binding Proteins (PBP’s)
- To bind to the PBPs, the β-lactam antibiotic must first diffuse through bacterial cell wall
- Gram -ve have an LPS layer that decreases antibiotic penetration
- cell wall is disrupted and lysis occurs
- results in a hypo-osmotic or iso-osmotic
- Active only against rapidly multiplying organisms
- Differences in broad vs narrow spectrum are due to affinity for PBPs
gram +ve more susceptible to β-lactams than gram-negative bacteria
Briefly describe what a bactericidal antibiotic is
- Kills 99.99% bacteria
- Inhibits cells wall synthesis
-usually beta lactams
-good for needing to get rid of bacteria quickly (meningitis)
Briefly describe what a bacteriostatic antibiotic is
*inhibit growth
*Inhibit protein synthesis, DNA replication or metabolism
How do bacteria become resistant to Penicillins and cephalosproins
Both types of antibiotic contain a beta lactam ring
Beta-lactamase hydrolysis this ring and the antibiotic and now unable to bing to the PBP’s
Why do bacteria develop resistance?
*Intrinsic - Naturally resistant
*Extrinsic - Naturally acquired through spontaneous gene mutation or horizontal gene transfer (conjunction, transduction, transformation)
what are the two major determinants of anti bacterial effects
concentration and the time
Describe intrinsic bacterial resistance.
D
Briefly describe Horizontal gene transfer: Transduction with and example.
*Insertion of DNA by bacteriophages
*mecA genes for MRSA
Briefly describe horizontal gene transfer: Conjugation and and example.
*Sharing of extra chromosomal DNA plasmids
*New Delhi metallo-B-lactamase