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Study 2nd sem > Bacte specimen management > Flashcards

Bacte specimen management Flashcards

1
Q

The goal of the collector in specimen collection

A

maintain the viability of
organisms with minimal contamination

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2
Q

collect the specimen in the ______________ of the infection _________________________ and _________________________________

A

acute (early) phase, or within 2-3 days for viral infections, before antibiotics are administered

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3
Q

Collect the specimen using the proper technique and
supplies with minimal contamination from ____________________

A

normal biota

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4
Q

Label the specimen accurately with the specific
anatomic site and the patient information—__________________________________________________

A

patient’s
name and unique identification number, as well as
date and time of collection.

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5
Q

collection medium for upper respiratory tract, and genitourinary tract

A

Pre-moistened Swab

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6
Q

collection medium for Stool

A

Clean Leak-proof
Container

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7
Q

collection medium for corneal scrapings

A

Bedside Plating

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8
Q

collection medium for Hair, Nails, Skin scrapings

A

Clean Screw Top-tube (for
fungal culture)

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9
Q

Bags should be marked with ______________________________

A

BIOHAZARD LABEL

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10
Q

when using swabs ________________ should be summited

A

Two swabs, (direct smear and culture)

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11
Q

Wound fibers
may trap
specimens and
prevent the
efficient release

A

Regular Fiber Swab

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12
Q

Perpendicular
arrangement of fibers
and solid swab head
aid in the release of
specimen

A

Nylon Flocked Swab

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13
Q

preferred material for the tip of swabs

A

Dacron, rayon, or Calcium alginate

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14
Q

contain excessive fatty acids
which are inhibitory and toxic to some bacteria

A

Cotton-tipped swabs

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15
Q

To counteract fatty acids, ___________________ can be added to media to absorb fatty acids present
in the specimen

A

charcoal

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16
Q

_______________________
are not recommended for viral collection (SARS-CoV-2)

A

Calcium alginate swabs or swabs with wooden shafts

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17
Q

is not an appropriate specimen source when the exact
anatomic site is not provided.

A

WOUND

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18
Q

swab recommended for viral collection

A

Use synthetic fiber swabs with thin or wire shafts (nylon flocked swabs

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19
Q

The specimen for wound should be collected by _______________________ rather than by swab from the advancing
margin of the lesion

A

needle aspiration

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20
Q

Specimen that can be collected by patients

A

Urine, sputum, stool

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21
Q

specimen should be Transported ideally within __________ of collection, preferably within _____

A

30 mins, 2 hrs

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22
Q

specimens that must immediately be transported

A

Bone, Prostatic samples tube

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23
Q

q
specimens that must transported in less than 15 mins

A

Body fluids, CSF, Gastric wash or lavage

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24
Q

specimens that must transported within 1 hour

A

Gastric biopsy, Stool

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25
Q

specimens that must transported within 24 hours

A

Bone marrow aspirate, preserved clean-voided midstream urine

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26
Q

q
fungal culture, hair, nail scrapings must be transported within ________

A

● 72 hrs

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27
Q

unpreserved urine must be transported within __________

A

30 mins

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28
Q

If transport is delayed, the specimen can be
maintained by storage under certain conditions or with
the use of

A

Preservatives
○ Anticoagulants
○ Transport or holding medium or culture
medium

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29
Q

preservation for
stool for ova and parasite examination ( maintain the
integrity of trophozoites and cysts)

A

Boric Acid

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30
Q

preservation for
stool for ova and parasite examination ( maintain the
integrity of trophozoites and cysts)

A

Polyvinyl Alcohol (PVA) and Buffered Formalin

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31
Q

__________ for Clostridium difficile toxin assay should be
collected without a preservative and can be
refrigerated

A

Stools

32
Q

Preservatives (fixatives) should NOT be ADDED to
fecal specimens for _________________

A

bacteriological testing

33
Q

Stool specimens for bacterial culture that are
not transported immediately can be _________________, at around

A

refrigerated, -70 deg C

34
Q

f the delay is longer than 2 hours, the specimen can be added to ___________________________

A

Cary-Blair transport medium

35
Q

Contains substances that do not promote the
multiplication of microorganisms but ensure their
preservation and are available in swab collection
systems

A

TRANSPORT OR HOLDING MEDIA

36
Q

Commercial transport
system used for N.
gonorrhoeae

A

JEMBEC (John E. Martin
Biologic Environmental
Chamber)

37
Q

Contains selective agar
(Modified Thayer-Martin)
and carbon dioxide
generating tablet (sodium
bicarbonate and citric
acid)

A

JEMBEC (John E. Martin
Biologic Environmental
Chamber)

38
Q

● Recovery of aerobic and
anaerobic bacteria
● For respiratory samples

A

Amie’s transport media

39
Q

● For stool pathogens
(Salmonella, Shigella,
Vibrio, or Campylobacter)

A

Cary-Blair

40
Q

Multi-purpose transport
media

A

Stuart’s

41
Q

● Neisseria

A

Transgrow

42
Q

● For vaginal swab (S.
agalactiae)

A

Todd-Hewitt and LIM
(Modified Todd-Hewitt)

43
Q

● Viral Transport Media
(VTM)

A

Leibovitz-Emory media

44
Q

● Suspected bacillary
dysentery (stool)

A

Sach’s buffered glycerol
saline

45
Q

● Suspected Cholera

A

Venkatraman
Ramakrishnan (VR)
medium

46
Q

To prevent clotting of specimens, including blood,
bone marrow, and synovial fluid ( if the specimen is
clotted, organisms are trapped in clotted material an
difficult to isolate)

A

ANTICOAGULANTS

47
Q

commonly used and is the most
appropriate/common anticoagulant used for
microbiology specimens

A

Sodium polyanethol sulfonate (SPS) at 0.025

48
Q

blood to sps ratio

A

10:1

49
Q

______________ - counteracts the action of SPS

A

Addition of 1% gelatin

50
Q

often used for viral cultures and isolation of
Mycobacterium spp. from blood and may inhibit the
growth of gram-positive bacteria and yeast

A

Heparin

51
Q

specimen storage for Body Fluids, Bone
IUD (Intrauterine Device)
IV catheters
Prostatic Fluid in TUBES
Suprapubic Aspirate

A

Must be plated as
soon as received

52
Q

specimen storage for Blood, Corneal Scrapings

A

Must be incubated at
37 deg C on

53
Q

specimen storage for blood

A

Less than or equal to
2 hrs at ROOM TEMP

54
Q

specimen storage for Abscess, Bone Marrow Aspirate,
Body fluids, Inner Ear, CSF for
bacteria (15th edition), Conjunctiva,
Aqueous Fluid, Corneal Scraping,
Rectal Swab, Genital Tracts,
Prostate fluid SWAB, Urethra,
Upper Respiratory Tract, TissuesWithin 24 hrs at
ROOM
TEMPERATURE

A

Within 24 hrs at
ROOM
TEMPERATURE

55
Q

specimen storage for Pericardial Fluid and other fluids
(fungal culture), External Ear, IV
Catheters, Gastric Wash/Lavage,
Gastric Biopsy, Stool (Routine),
Unpreserved stool for E. coli toxin
assays, BAL, Endotracheal
aspirate, BW/BB, Sputum,
Clean-voided midstream urine and
Straight catheter

A

Within 24 hrs at 4 deg C

56
Q

specimen storage for C. difficile culture or nucleic acid
detection

A

2 days at 4 deg C

57
Q

q
specimen storage for CSF for viruses detection

A

3 days at 4 C

58
Q

specimen storage for Stool for O&P Examination, Hair,
Skin and Nails (for Fungal Culture)

A

INDEFINITELY at
Room Temperature

59
Q

Layers of triple package

A

PRIMARY RECEPTACLE
SECONDARY CONTAINER
THIRD RECEPTACLE

60
Q

contains the infectious
substance and the receptacle should be
watertight, leak-proof, and labeled properly
including the contents of the sample

A

Primary receptacle

61
Q

watertight,
leakproof packaging, and is used to enclose
and protect the primary receptacle(s)

A

Secondary container

62
Q

protects the secondary
container from physical damage during
transport (rigid outer packaging)

A

Third receptacle

63
Q

a material known or
suspected to contain a pathogen (bacteria, viruses,
rickettsiae, parasites, fungi, or prions) that causes
disease in humans or animals.

A

infectious substances

64
Q

Critical/Invasive
- Potentially
life-threatening and
from an invasive
source
- Require immediate
processing

A

LEVEL 1 SPECIMEN

65
Q

Amniotic fluid,
Blood, Brain,
CSF, Heart
valves,
Pericardian fluid

A

LEVEL 1 SPECIMEN

66
Q

Unpreserved
- Unprotected and
may quickly
degrade or have
overgrowth of
contaminating biota

A

LEVEL 2 SPECIMEN

67
Q

Body fluids (not
listed for Level 1).
Bone, Drainage
from wounds,
Feces, Sputum,
Tissue

A

LEVEL 2 SPECIMEN

68
Q

specimen with Quantitation required

A

LEVEL 3 SPECIMEN

69
Q

Feces and urine
in preservative,
swabs in holding
medium

A

LEVEL 4 SPECIMEN

70
Q

Preserved specimen

A

LEVEL 4 SPECIMEN

71
Q

Proper identification of each specimen includes a
label firmly attached to the container with the following
information:

A

Name
○ ID number
○ Room number
○ Physician
○ Culture Site
○ Date of Collection
○ Time of Collection

72
Q

The ____________________ must contain sufficient
information for the specimen and requisition to be
matched up when received in the laboratory

A

specimen label

73
Q

Blood should be drawn during the time of _______________

A

febrile (fever) episode.

74
Q

The venipuncture site should be disinfected with
______________________________

A

Chlorhexidine-alcohol. (70 % Alcohol then 2%
clorhexidine then 2% tincture of iodine)

75
Q

is the
recommended skin antiseptic for blood
cultures, infants 2 months and older and for
patients with iodine sensitivity.

A

Chlorhexidine gluconate

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