Bacte LAB Sterilization w/ Environmental monitoring

Question 1

describes a process that destroys or eliminates all forms of
microbial life and is carried out in health-care facilities by physical or
chemical methods.

Answer 1

STERILIZATION

Question 2

describes a process that eliminates many or all pathogenic
microorganisms, except bacterial spores, on inanimate objects. In
health-care settings, objects usually are disinfected by liquid chemicals or
wet pasteurization. Each of the various factors that affect the efficacy of
disinfection can nullify or limit the efficacy of the process.

Answer 2

DISINFECTION

Question 3

A process of washing, cleaning, or removing dirt, and eliminates dust, debris, and germs on the surface. It doesn’t kill germs, but by removing them, it lowers their numbers and risk of infection

Answer 3

SANITIZING

Question 4

a process of destroying all forms of microbial life and is carried out in medical facilities by physical or chemical methods

Answer 4

STERILIZING

Question 5

using chemicals to kill germs on surfaces. This process doesn’t necessarily clean dirty surfaces or remove germs, but killing forms on a surface after cleaning, can further lower the risk of opening

Answer 5

DISINFECTING

Question 6

agent used to disinfect inanimate objects or surfaces but is generally toxic
to use on human tissues

Answer 6

Disinfectant

Question 7

gent that kills or inhibits growth of microbes but is safe to use on human
tissue.

Answer 7

Antiseptics

Question 8

agent that reduces, but may not eliminate, microbial numbers to a safe level.

Answer 8

Sanitizer

Question 9

something (such as an antiseptic or disinfectant) that destroys or inactivates
pathogens (such as bacteria, viruses, and fungi)

Answer 9

Germicide

Question 10

CHEMICAL METHODS OF STERILIZATION (3)

Answer 10

Heat Sterilization, filtration, irradiation

Question 11

CHEMICAL METHODS OF STERILIZATION

Gaseous Sterilization - (4)

Answer 11

Ethylene oxide, Formaldehyde, Nitrogen dioxide, Ozone

Question 12

CHEMICAL METHODS OF STERILIZATION

Liquid Sterilization

Answer 12

Liquid Sterilization
-Hydrogen peroxide
- Glutaraldehyde
- Hypochlorite

Question 13

Most EPA-registered hospital
disinfectants have a label contact time of ________________________. However, many
scientific studies have demonstrated
the efficacy of hospital disinfectants
against pathogens with a contact time of at least _________________

Answer 13

10 minutes, 1 minute.

Question 14

Microbial communities that are tightly attached to surfaces
and cannot be easily removed

Answer 14

Biofilm

Question 15

Factors affecting the efficacy of disinfection and sterilization (7)

Answer 15

Number and Location of Microorganisms

Innate Resistance of Microorganisms

Concentration and Potency of Disinfectants

Physical and Chemical Factors

Organic and Inorganic matter

Duration of exposure

Biofilms

Question 16

A program designed to demonstrate the control of viable and non-viable
particles in critical areas.

Answer 16

MICROBIAL MONITORING

Question 17

Testing for the detection and enumeration of bacteria, yeast, and mold. It
includes the monitoring of personnel, air and area surfaces for microbial
contamination

Answer 17

VIABLE MONITORING

Question 18

A reference for particle counts measured by a laser counter.

Answer 18

NON-VIABLE MONITORIN

Question 19

Describes the processes and activities that need to take place to characterize
and monitor the quality of the environment

Answer 19

ENVIRONMENTAL MONITORING

Question 20

Talking for 5 minutes and coughing each
can produce _____________________; sneezing can generate approximately
40,000 droplets which then evaporate to particles in the size range of 0.5–12
μm.

Answer 20

3,000 droplet nuclei, 40,000 droplets

Question 21

is used as needed to determine the
numbers and types of microorganisms, or particulates, in indoor air.

Answer 21

Microbiological air sampling

Question 22

Expose settle plate for ________ and incubate for ____ at _____________

Answer 22

60 minutes
24 hours, 35 deg C