Bacte LAB Sterilization w/ Environmental monitoring Flashcards
describes a process that destroys or eliminates all forms of
microbial life and is carried out in health-care facilities by physical or
chemical methods.
STERILIZATION
describes a process that eliminates many or all pathogenic
microorganisms, except bacterial spores, on inanimate objects. In
health-care settings, objects usually are disinfected by liquid chemicals or
wet pasteurization. Each of the various factors that affect the efficacy of
disinfection can nullify or limit the efficacy of the process.
DISINFECTION
A process of washing, cleaning, or removing dirt, and eliminates dust, debris, and germs on the surface. It doesn’t kill germs, but by removing them, it lowers their numbers and risk of infection
SANITIZING
a process of destroying all forms of microbial life and is carried out in medical facilities by physical or chemical methods
STERILIZING
using chemicals to kill germs on surfaces. This process doesn’t necessarily clean dirty surfaces or remove germs, but killing forms on a surface after cleaning, can further lower the risk of opening
DISINFECTING
agent used to disinfect inanimate objects or surfaces but is generally toxic
to use on human tissues
Disinfectant
gent that kills or inhibits growth of microbes but is safe to use on human
tissue.
Antiseptics
agent that reduces, but may not eliminate, microbial numbers to a safe level.
Sanitizer
something (such as an antiseptic or disinfectant) that destroys or inactivates
pathogens (such as bacteria, viruses, and fungi)
Germicide
CHEMICAL METHODS OF STERILIZATION (3)
Heat Sterilization, filtration, irradiation
CHEMICAL METHODS OF STERILIZATION
Gaseous Sterilization - (4)
Ethylene oxide, Formaldehyde, Nitrogen dioxide, Ozone
CHEMICAL METHODS OF STERILIZATION
Liquid Sterilization
Liquid Sterilization
-Hydrogen peroxide
- Glutaraldehyde
- Hypochlorite
Most EPA-registered hospital
disinfectants have a label contact time of ________________________. However, many
scientific studies have demonstrated
the efficacy of hospital disinfectants
against pathogens with a contact time of at least _________________
10 minutes, 1 minute.
Microbial communities that are tightly attached to surfaces
and cannot be easily removed
Biofilm
Factors affecting the efficacy of disinfection and sterilization (7)
Number and Location of Microorganisms
Innate Resistance of Microorganisms
Concentration and Potency of Disinfectants
Physical and Chemical Factors
Organic and Inorganic matter
Duration of exposure
Biofilms
A program designed to demonstrate the control of viable and non-viable
particles in critical areas.
MICROBIAL MONITORING
Testing for the detection and enumeration of bacteria, yeast, and mold. It
includes the monitoring of personnel, air and area surfaces for microbial
contamination
VIABLE MONITORING
A reference for particle counts measured by a laser counter.
NON-VIABLE MONITORIN
Describes the processes and activities that need to take place to characterize
and monitor the quality of the environment
ENVIRONMENTAL MONITORING
Talking for 5 minutes and coughing each
can produce _____________________; sneezing can generate approximately
40,000 droplets which then evaporate to particles in the size range of 0.5–12
μm.
3,000 droplet nuclei, 40,000 droplets
is used as needed to determine the
numbers and types of microorganisms, or particulates, in indoor air.
Microbiological air sampling
Expose settle plate for ________ and incubate for ____ at _____________
60 minutes
24 hours, 35 deg C
