Bacte Nutrition Flashcards
MAJOR nutritional needs of bacteria
Carbon, Nitrogen, ATP
responsible for making cellular components and representing 50% of the dry weight of the bacterium
CARBON
responsible for making proteins and makes up 14% of the bacterium
NITROGEN
NITROGEN
ATP
This makes up the additional 4% of the weight of the bacteria
Phosphates for nucleic acid
Phospholipids for cell membranes
Sulfur for protein synthesis
use carbon dioxide as sole carbon source, with only water and inorganic salts as substrates
Autotrophs
reduce in inorganic molecules for energy conservation/ biosynthesis
litotrophs
require an organic source of carbon (glucose) and obtain energy by either oxidation or fermentation
Heterotrophs
require organic substance for growth and multiplication
organotrophs
obtain energy photosynthetically
phototrophs
obtain energy through oxidation of inorganic compounds
Chemolithotrophs
all bacteria that inhabit the human body belong to this category
Heterotrophs
organisms that require oxygen and grow well with room air
Aerobes
Aerobes grows in ambient air, which contains ____ oxygen and a small amount ____ of CO2
21%, 0.03%/ 0.04% (mahon book)
do not require oxygen to grown and survive
Anaerobes
cannot grow in the presence of oxygen
Anaerobes
atmosphere of anaerobe jars, bags, or chambers is composed of _________ hydrogen, _______ CO2, __________ nitrogen, and ____ 02
5-10% Hydrogen
5-10% CO2
80-90% Nitrogen
0% O2
do not require the presence of oxygen, and they die after prolonged exposure to air
Obligate Anaerobes
Example of Obligate Anaerobes
Clostridium and Bacteroides
most clinically significant bacteria, organisms that can grow either in the presence or absence of oxygen
Facultative Anaerobes
aerobes that can grow anearobically
Facultative Anaerobes
Example of Facultative Anaerobes
Enterobacteriaceae
organisms can survive the presence of oxygen but unable to perform metabolic processes unless situated in an anaerobic environment
Aerotolerant Anaerobes
example of Aerotolerant Anaerobes
Lactobacillus and Cutibacterium
most potent biological oxidant known, and detrimental to cell components such as proteins and nucleic acids
Hydroxyl radical
why some organisms are anearobic
lack enzymes to counteract superoxide anion and hydrogen peroxide
Obligate aerobes and facultative anaerobic bacteria have ____________ and _____________
Superoxide dismutase, catalase
converts the superoxide anion to oxygen and H2O2
Superoxide dismutase
breaks H2O2 to O2 and water
Catalase
an organism that requires only 2-10% oxygen (Decreased Oxygen) for growth and increased CO2 (8-10%)
Microaerophiles
EXAMPLE of Microaerophiles
Campylobacter, Helicobacter, Treponema
An organism that requires increased CO2 (5-10%) and approximately 15% O2
Capnophiles
Capnophiles examples
Streptococcus pneumoniae, Neisseria gonorrhoeae
grows well at 1-20 deg C (ave: 10 deg C)
Psychrophiles/ Cryophiles
Psychrophiles/ Cryophiles example
Listeria monocytogenes, Yersinia enterocolitica
Grow between at 20 to 45 deg C
Mesophiles
most commonly encountered pathogenic bacteria
Mesophiles
the optimum temperature for most clinically significant bacteria is _________________
35-37deg C
organisms grow at 50-60 deg C
Thermophiles/ Hyperthermophiles
Thermophiles/ Hyperthermophiles example
Geobacillus stearothermophilus, Thermus aquaticus
prokaryotes that are able to survive in unusual conditions like the absence of oxygen. increased temperature, and living below the surface of the Earth
Extremophiles
Extremophiles example
Bacillus infernus
measure of the hydrogen ion concentration
pH Scale
the optimum pH for most pathogenic bacteria is from pH ______________________
6.5 to 7.5
Diagnostic laboratory culture media for bacterial isolation are usually adjusted to a final pH between _____________________
7.0 to 7.5
grow between pH 0-5.5
Acidophiles
grow between pH 5.5-8.0
Neutrophiles
grown between 8.5- 11.5
Alkalophiles
examples for Acidophiles
Lactobacillus acidophilus
most clinically significant bacteria according to pH requirement
neutrophiles
examples for Alkalophiles
Vibrio
this is indispensable for bacterial growth. it serves as a solvent for food and forms the major portion of the protoplasm
moisture
organisms requiring increased moisture content are termed __________________________
humidophiles
organisms that require and grown in increased concentration of sodium chloride
Halophiles
Halophiles examples
listeria monocytogenes, Staphylococcus aureus, Vibrio spp.
are substances that are required by fastidious bacteria for their growth and multiplication
growth factors
organisms that require complex nutritional requirement/ growth factors in order to grow (blood)
Fastidious
examples of growth factors
Amino acids
Purines
Pyrimidines
Vitamins
Fatty Acids
growth factor for h. influenzae
blood
oxidized form of hemoglobin
X factor AKA hemin (heat stable)
is produced by some bacteria or from potato or yeast extract
V factor AKA NAD/ Coenzyme I (Heat liable)
Bacteria according to carbon source
AUTOTROPHS
HETEROTROPHS
Bacteria according to oxygen requirement
AEROBES
ANAEROBES
MICROAEROPHILES
TYPES OF ANAEROBES
OBLIGATE ANAEROBES
FACULTATIVE ANAEROBES
AEROTOLERANT ANAEROBES
Bacteria according to CO2 requirement
CAPNOPHILES
Bacteria according to temperature requirement
-PSYCHROPHILES/ CRYPHILES
-MESOPHILES
-THERMOPHILES/ HYPERTHERMOPHILES
-EXTREMOPHILES
Bacteria according to pH requirement
ACIDOPHILES
NEUTROPHILES
ALKALOPHILES
Bacteria according to moisture requirement
HUMIDOPHILES
Bacteria according to inorganic salts concentration requirements
HALOPHILES
Bacteria according to growth factors
FASTIDIOUS