9. Family Enterobacteriaceae (I) Flashcards

1
Q

Microscopic appearance of the family Enterobacteriaceae

A

Gram-negative bacilli

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2
Q

Oxygen requirements for the family Enterobacteriaceae

A

Facultative anaerobes

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3
Q

Colony morphology for most Enterobacteriaceae

A

Large
Gray
Spreading colonies

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4
Q

2 genera of the family Enterobacteriaceae that generally differs in colony morphology

A

Klebsiella

Enterobacter

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5
Q

Colony morphology of Klebsiella and Enterobacter

A

Mucoid

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6
Q

Carbohydrate fermented by all Enterobacteriaceae

A

Glucose

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7
Q

Nitrate reduction characteristics of Enterobacteriaceae

A

Reduces nitrates to nitrites (with rare exceptions)

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8
Q

Oxidase reaction of Enterobacteriaceae

A

Oxidase negative

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9
Q

Motility of Enterobacteriaceae

A

Most are motile by peritrichous flagella

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10
Q

Cell-associated antigens of the family Enterobacteriaceae

A

O: somatic antigen (heat stable)
K: capsular antigen (heat labile)
H: flagellar antigen (heat labile)

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11
Q

Cell-associated antigen associated with endotoxin release

A

O

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12
Q

Which cell-associated antigen when present, causes the strains to be more pathogenic?

A

K

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13
Q

Cell-associated antigen responsible for motility

A

H

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14
Q

Which cell-associated antigen(s) is/are heat stable?

A

O

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15
Q

Which cell-associated antigen(s) is/are heat labile?

A

K

H

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16
Q

Which of the Enterobacteriaceae are primary intestinal pathogens?

A

Plesiomonas
Salmonella
Shigella
Yersinia enterocolitica

PSSY

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17
Q

Which of the Enterobacteriaceae are opportunistic?

A
Proteus
Providencia
Escherichia
Morganella
Citrobacter
Hafnia
Edwardsiella
Enterobacter
Klebsiella
Serratia

PPEMCHEEKS

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18
Q

Media used to recover Enterobacteriaceae

A

MacConkey agar
XLD
Hektoen

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19
Q

Carbohydrate used for initial differentiation of Enterobacteriaceae

A

Lactose

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20
Q

Food-borne enteric pathogens

A

Salmonella
E. coli
Campylobacter
Yersinia

SECY

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21
Q

Human to human enteric pathogens

A

Shigella
Salmonella typhi
Helicobacter pylori

SSH

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22
Q

Water-borne enteric pathogens

A

Vibrio
Aeromonas
Plesiomonas

VAP

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23
Q

Salmonella heat sensitive antigens for serotyping

A

H antigens

24
Q

Salmonella virulence antigens for serotyping

A

Vi (K) antigens

25
Q

Salmonella antigens that may mask O antigens

A

Vi (K) antigens

26
Q

How to remove Vi (K) antigens so that it will not mask O antigens?

A

Boiling

27
Q

Most common illness caused by Salmonella

A

Gastroenteritis

28
Q

Salmonella spp. that causes bacteremia/septicemia

A

Salmonella choleraesuis

29
Q

Disease caused by S. typhi

A

Enteric fever: Typhoid fever

30
Q

Culture(s) used to diagnose S. typhi infection

A

Blood culture: + during first week

Stool culture: + during second week

31
Q

Culture(s) used to diagnose gastroenteritis caused by Salmonella

A

Positive stool cultures

32
Q

Culture used to diagnose infection with S. choleraesuis

A

Positive blood culture

33
Q

Disease caused by S. choleraesuis

A

Bacteremia/septicemia

34
Q

Serotyping of Shigella species based on O antigen

A

A: S. dysenteriae
B: S. flexneri
C: S. boydii
D: S. Sonnei

35
Q

Which Shigella spp. are physiologically similar?

A

S. dysenteriae (A)
S. flexneri (B)
S. boydii (C)

36
Q

How do you differentiate S. sonnei (Group D) from Groups A-C?

A

Biochemical tests

37
Q

MOT of Shigella spp.

A

Fecal-oral route (human to human)

38
Q

True/False. Shigella spp. are vulnerable to gastric acidity.

A

False. Shigella spp. are resistant to gastric acids

39
Q

Toxin by Shigella spp. that causes inflammation and ulcerative lesions

A

Shiga toxin (cytotoxin)

40
Q

Lactose ferementation characterictics of Shigella spp.

A

Lactose negative

41
Q

TSI characteristics of Shigella spp.

A

Alkaline/acid
No gas
No H2S

42
Q

Urease characteristics of Shigella spp.

A

Urease negative

43
Q

Most important E. coli

A

Enterohemorrhagic E. coli (EHEC)

44
Q

Enterohemorrhagic E. coli is also known as

A

E. coli O157:H7

45
Q

Carbohydrates fermented by E. coli

A

Glucose
Lactose
Xylose

46
Q

Indole reaction of E. coli

A

Indole positive

47
Q

Methyl Red (MR) reaction of E. coli

A

MR positive

48
Q

H2S production of E. coli

A

No H2S

49
Q

Urease production of E. coli

A

No urease

50
Q

Citrate reaction of E. coli

A

Citrate negative

51
Q

Media used for E. coli O157:H7

A

MacConkey with Sorbitol (SMAC)

52
Q

Colony morphology of E. coli on SMAC

A

Clear (does not ferment sorbitol)

53
Q

Lactose fermentation characteristics of Salmonella

A

Lactose negative

54
Q

H2S production of Salmonella

A

H2S positive

55
Q

The family Enterobacteriaceae is normal flora in the

A

GI tract