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Elsevier Bacteriology (RVSP) > 9. Family Enterobacteriaceae (I) > Flashcards

9. Family Enterobacteriaceae (I) Flashcards

1
Q

Microscopic appearance of the family Enterobacteriaceae

A

Gram-negative bacilli

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2
Q

Oxygen requirements for the family Enterobacteriaceae

A

Facultative anaerobes

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3
Q

Colony morphology for most Enterobacteriaceae

A

Large
Gray
Spreading colonies

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4
Q

2 genera of the family Enterobacteriaceae that generally differs in colony morphology

A

Klebsiella

Enterobacter

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5
Q

Colony morphology of Klebsiella and Enterobacter

A

Mucoid

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6
Q

Carbohydrate fermented by all Enterobacteriaceae

A

Glucose

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7
Q

Nitrate reduction characteristics of Enterobacteriaceae

A

Reduces nitrates to nitrites (with rare exceptions)

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8
Q

Oxidase reaction of Enterobacteriaceae

A

Oxidase negative

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9
Q

Motility of Enterobacteriaceae

A

Most are motile by peritrichous flagella

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10
Q

Cell-associated antigens of the family Enterobacteriaceae

A

O: somatic antigen (heat stable)
K: capsular antigen (heat labile)
H: flagellar antigen (heat labile)

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11
Q

Cell-associated antigen associated with endotoxin release

A

O

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12
Q

Which cell-associated antigen when present, causes the strains to be more pathogenic?

A

K

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13
Q

Cell-associated antigen responsible for motility

A

H

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14
Q

Which cell-associated antigen(s) is/are heat stable?

A

O

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15
Q

Which cell-associated antigen(s) is/are heat labile?

A

K

H

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16
Q

Which of the Enterobacteriaceae are primary intestinal pathogens?

A

Plesiomonas
Salmonella
Shigella
Yersinia enterocolitica

PSSY

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17
Q

Which of the Enterobacteriaceae are opportunistic?

A 
Proteus
Providencia
Escherichia
Morganella
Citrobacter
Hafnia
Edwardsiella
Enterobacter
Klebsiella
Serratia

PPEMCHEEKS

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18
Q

Media used to recover Enterobacteriaceae

A

MacConkey agar
XLD
Hektoen

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19
Q

Carbohydrate used for initial differentiation of Enterobacteriaceae

A

Lactose

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20
Q

Food-borne enteric pathogens

A

Salmonella
E. coli
Campylobacter
Yersinia

SECY

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21
Q

Human to human enteric pathogens

A

Shigella
Salmonella typhi
Helicobacter pylori

SSH

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22
Q

Water-borne enteric pathogens

A

Vibrio
Aeromonas
Plesiomonas

VAP

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23
Q

Salmonella heat sensitive antigens for serotyping

A

H antigens

24
Q

Salmonella virulence antigens for serotyping

A

Vi (K) antigens

25
Salmonella antigens that may mask O antigens
Vi (K) antigens
26
How to remove Vi (K) antigens so that it will not mask O antigens?
Boiling
27
Most common illness caused by Salmonella
Gastroenteritis
28
Salmonella spp. that causes bacteremia/septicemia
Salmonella choleraesuis
29
Disease caused by S. typhi
Enteric fever: Typhoid fever
30
Culture(s) used to diagnose S. typhi infection
Blood culture: + during first week | Stool culture: + during second week
31
Culture(s) used to diagnose gastroenteritis caused by Salmonella
Positive stool cultures
32
Culture used to diagnose infection with S. choleraesuis
Positive blood culture
33
Disease caused by S. choleraesuis
Bacteremia/septicemia
34
Serotyping of Shigella species based on O antigen
A: S. dysenteriae B: S. flexneri C: S. boydii D: S. Sonnei
35
Which Shigella spp. are physiologically similar?
S. dysenteriae (A) S. flexneri (B) S. boydii (C)
36
How do you differentiate S. sonnei (Group D) from Groups A-C?
Biochemical tests
37
MOT of Shigella spp.
Fecal-oral route (human to human)
38
True/False. Shigella spp. are vulnerable to gastric acidity.
False. Shigella spp. are resistant to gastric acids
39
Toxin by Shigella spp. that causes inflammation and ulcerative lesions
Shiga toxin (cytotoxin)
40
Lactose ferementation characterictics of Shigella spp.
Lactose negative
41
TSI characteristics of Shigella spp.
Alkaline/acid No gas No H2S
42
Urease characteristics of Shigella spp.
Urease negative
43
Most important E. coli
Enterohemorrhagic E. coli (EHEC)
44
Enterohemorrhagic E. coli is also known as
E. coli O157:H7
45
Carbohydrates fermented by E. coli
Glucose Lactose Xylose
46
Indole reaction of E. coli
Indole positive
47
Methyl Red (MR) reaction of E. coli
MR positive
48
H2S production of E. coli
No H2S
49
Urease production of E. coli
No urease
50
Citrate reaction of E. coli
Citrate negative
51
Media used for E. coli O157:H7
MacConkey with Sorbitol (SMAC)
52
Colony morphology of E. coli on SMAC
Clear (does not ferment sorbitol)
53
Lactose fermentation characteristics of Salmonella
Lactose negative
54
H2S production of Salmonella
H2S positive
55
The family Enterobacteriaceae is normal flora in the
GI tract

Elsevier Bacteriology (RVSP) (34 decks)

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