1. Intro (II)

Question 1

Fermentable sugars in HEA

Answer 1

Lactose

Sucrose

Question 2

pH indicators in HEA

Answer 2

Bromophenol blue

Acid fuchsin

Question 3

Component of HEA; detects H2S production

Answer 3

Ferric ammonium citrate

Question 4

HEA is differential for

Answer 4

Salmonella and Shigella

Question 5

Characteristics of lactose fermenters in HEA

Answer 5

Yellow-orange colonies (E. coli)

Question 6

Characteristics of non-lactose fermenters in HEA

Answer 6

Colorless/green colonies with unchanged medium (Shigella, Providencia)

Question 7

Characteristics of H2S producers in HEA

Answer 7

Black colonies (Salmonella)

Question 8

CNA inhibits

Answer 8

Most gram-negative organisms

Question 9

Purpose of phenylethyl alcohol in PEA

Answer 9

Inhibits gram-negative organisms

Question 10

3 key media for routine anaerobic organisms

Answer 10

Blood agar
Supplemented PEA
Kanamycin-vancomycin-laked blood agar (K-V agar)

Question 11

Usual agar base for Blood Agar

Answer 11

Trypticase soy

Brain heart infusion

Question 12

A good nonselective medium for initial isolation of anaerobes

Answer 12

Blood Agar

Question 13

Agar that is selective for anaerobes

Answer 13

Supplemented PEA

Question 14

2 components added to PEA to make it selective for anaerobes

Answer 14

Vitamin K

Hemin

Question 15

Function of Vitamin K and hemin in Supplemented PEA

Answer 15

Suppresses growth of facultative gram-negative bacilli

Question 16

Agar that is selective for gram-negative anaerobic bacilli

Answer 16

Kanamycin-Vancomycin-laked blood agar (K-V agar)

Question 17

Component of K-V agar that helps separate aerobes and anaerobes in mixed cultures

Answer 17

Aminoglycoside

Question 18

Components of K-V agar that permits growth of only gram-negative anaerobes

Answer 18

Kanamycin and Vancomycin

Question 19

Agar that limits the swarming of Proteus spp.

Answer 19

K-V agar

Question 20

Broth medium that serves as a check for plates when the primary shows no growth

Answer 20

Back-up Broth

Question 21

Use of back-up broth should be limited to ______ to _______

Answer 21

Fluids

Tissue

Question 22

Atmospheric requirements for microaerophilic organisms

Answer 22

5% O2
10% CO2
85% N

Question 23

Atmospheric requirements for anaerobes

Answer 23

85% N2
10% H2
5% CO2

Question 24

Component of HEA that inhibits gram-positive organisms

Answer 24

Bile salts