1. Intro (II) Flashcards
Fermentable sugars in HEA
Lactose
Sucrose
pH indicators in HEA
Bromophenol blue
Acid fuchsin
Component of HEA; detects H2S production
Ferric ammonium citrate
HEA is differential for
Salmonella and Shigella
Characteristics of lactose fermenters in HEA
Yellow-orange colonies (E. coli)
Characteristics of non-lactose fermenters in HEA
Colorless/green colonies with unchanged medium (Shigella, Providencia)
Characteristics of H2S producers in HEA
Black colonies (Salmonella)
CNA inhibits
Most gram-negative organisms
Purpose of phenylethyl alcohol in PEA
Inhibits gram-negative organisms
3 key media for routine anaerobic organisms
Blood agar
Supplemented PEA
Kanamycin-vancomycin-laked blood agar (K-V agar)
Usual agar base for Blood Agar
Trypticase soy
Brain heart infusion
A good nonselective medium for initial isolation of anaerobes
Blood Agar
Agar that is selective for anaerobes
Supplemented PEA
2 components added to PEA to make it selective for anaerobes
Vitamin K
Hemin
Function of Vitamin K and hemin in Supplemented PEA
Suppresses growth of facultative gram-negative bacilli
Agar that is selective for gram-negative anaerobic bacilli
Kanamycin-Vancomycin-laked blood agar (K-V agar)
Component of K-V agar that helps separate aerobes and anaerobes in mixed cultures
Aminoglycoside
Components of K-V agar that permits growth of only gram-negative anaerobes
Kanamycin and Vancomycin
Agar that limits the swarming of Proteus spp.
K-V agar
Broth medium that serves as a check for plates when the primary shows no growth
Back-up Broth
Use of back-up broth should be limited to ______ to _______
Fluids
Tissue
Atmospheric requirements for microaerophilic organisms
5% O2
10% CO2
85% N
Atmospheric requirements for anaerobes
85% N2
10% H2
5% CO2
Component of HEA that inhibits gram-positive organisms
Bile salts
