10. Miscellaneous Enteric Pathogens Flashcards
Oxygen requirements for Campylobacter spp.
Microaerophilic
Temperature where Arcobacter spp. grow
15-30°C
Variable form: 35-42°C
Oxygen requirements for Arcobacter spp.
Aerobic
Disease caused by Campylobacter jejuni
Food-borne gastroenteritis
What structure in the GI tract does C. jejuni attach to?
Gut enterocytes
Toxins released by C. jejuni
Enterotoxin
Cytotoxin
Temperature for growth of C. jejuni
42°C
Atmospheric conditions for growth of C. jejuni
5% O2
10% CO2
85% N2
Media used for C. jejuni
Campy blood agar
Other selective media for C. jejuni
- Cefoperazone Vancomycin Amphotericin (CVA)
- Skirrow Medium
Microscopic appearance of C. jejuni
Curved gram-negative bacilli
Sea gull shape
Colony morphology C. jejuni
Flat
Gray
Mucoid
Nonhemolytic colonies
Oxidase reaction of C. jejuni
Oxidase positive
Catalase reaction of C. jejuni
Catalase positive
Motility of C. jejuni in wet preparation
Darting motility
Hippurate hydrolysis reaction of C. jejuni
Hippurate hydrolysis positive
Bacteria that was previously classified as Vibrio and shares ecology of the family Vibrionaceae
Photobacterium damselae
Oxidase reaction of the Vibrio family
Oxidase positive
Oxygen requirements of the Vibrio family
Facultative anaerobe
Microscopic appearance of the Vibrio family
Gram-negative bacilli
Comma-shaped cells
The zooplankton which Vibrio cholerae is primarily attached to
Copepods
When V. cholerae colonizes the GI tract, it attaches to the
Villi using its pili
Two-part toxin produced by V. cholerae
Cholera toxin
Clinical disease caused by V. cholerae
Cholera
Lactose fermentation characteristics of V. cholerae
Non-lactose fermenter
Color of V. cholerae on TCBS agar
Yellow-green colonies
Oxidase reaction of V. cholerae
Oxidase positive
Catalase reaction of V. cholerae
Catalase positive
String test reaction of V. cholerae
String test positive
Enumerate the halophilic Vibrio organisms
V. parahaemolyticus
V. vulnificus
V. alginolyticus
Disease caused by V. parahaemolyticus
Gastroenteritis from seafood
Disease caused by V. vulnificus
Septicemia from raw shellfish (lactose fermenter)
Disease caused by V. alginolyticus
Wound and ear infections
Cytotoxic enterotoxin produced by Aeromonas hydrophila
Aerolysin
Hemolysis characteristics of Aeromonas hydrophila
β-hemolysis
Microscopic appearance of Aeromonas hydrophila
Gram-negative bacilli (straight)
Lactose fermentation characteristics of A. hydrophila
Lactose fermenter
Oxidase reaction of A. hydrophila
Oxidase positive
Indole reaction of A. hydrophila
Indole positive
Associated with gastric cancer
Helicobacter
Produced by Helicobacter; converts urea to ammonia and bicarbonate
Urease
Functions of ammonia
- neutralizes stomach acid
- toxic to epithelial cells
Enzymes produced by Helicobacter
- protease
- catalase
- phospholipases
Oxygen requirements of Helicobacter
Microaerophilic (5% O2, 10% CO2, 85% N2)
Temperature requirements for Helicobacter
35°C with high humidity
Oxidase reaction of Helicobacter
Oxidase positive
Catalase reaction of Helicobacter
Catalase positive
Urease reaction of Helicobacter
Urease positive
The urease produced by Helicobacter converts urea to
Ammonia
Bicarbonate
Genera under miscellaneous enteric pathogens
Campylobacter
Vibrio
Aeromonas
Helicobacter