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Elsevier Bacteriology (RVSP) > 25. Family Leptospiraceae > Flashcards

25. Family Leptospiraceae Flashcards

1
Q

Causative agent of Lyme disease

A

Borrelia burgdorferi

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2
Q

Causative agent of relapsing fever

A

Borrelia hermsii

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3
Q

Causative agent of Syphilis

A

Treponema pallidum

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4
Q

Disease caused by other treponemes other than T. pallidum

A

Yaws
Pinta
Bejel

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5
Q

Genera under the Family Leptospiraceae

A

Borrelia
Leptospira
Treponema

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6
Q

Microscopic appearance of Leptospira spp.

A

Coiled rods with hook

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7
Q

Oxygen requirements of Leptospira spp.

A

Obligate aerobes

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8
Q

Specimens cultured for Leptospirosis diagnosis

A

Blood (early)

Urine (late, >2 weeks)

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9
Q

Molecular diagnosis of Leptospirosis

A

PCR

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10
Q

Serological diagnosis of Leptospirosis

A

Enzyme immunoassay (EIA)

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11
Q

Oxygen requirements of Borrelia spp.

A

Microaerophilic

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12
Q

Optimum temperature for growth of Borrelia

A

28-30°C

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13
Q

Determines the helical shape of Borrelia spp.

A

Flagella

Flagella-negative mutants are straight bacilli

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14
Q

Disease caused by B. burgdorferi

A

Lyme borreliosis

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15
Q

Vectors for Lyme borreliosis

A
  • Ixodes scapularis: deer tick/black-legged tick

- Ixodes pacificus

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16
Q

Stages of Lyme disease

A 
Stage 1 (3-30 days)
- 75% develop erythema chronicum migrans
Stage 2 (1-7 months)
- cardiac and neurologic problems
Stage 3 (months to years)
- arthritis
17
Q

Explain the Western Blot diagnosis of Lyme borreliosis

A

POS IgM: 2 of 3 protein bands

POS IgG: 5 of 10 protein bands

18
Q

Disease caused by T. pallidum

A

Syphilis

19
Q

MOT of T. pallidum

A

Direct contact with lesions that contain T. pallidum

20
Q

Stages of syphilis

A

Primary: chancre
Secondary: rash
Latent
Tertiary: gummas/neurosyphilis

21
Q

Diagnostic test for primary syphilis

A

Dark-field microscopy

22
Q

Stages of syphilis when it is infectious

A

Primary
Secondary
Latent (early)

23
Q

Diagnostic tests for secondary syphilis

A

Dark-field microscopy (early)

Serological tests

24
Q

Diagnosis of tertiary syphilis

A

Serological tests

25
Q

In utero infection of T. pallidum

A

Congenital syphilis

26
Q

Manifestations of tertiary syphilis

A
  • gummatous syphilis
  • cardiovascular disease
  • neurosyphilis
27
Q

Examples of non-treponemal tests

A

RPR

VDRL

28
Q

Examples of treponemal tests

A

TP-PA

EIA

29
Q

Stain for all spirochetes

A

Silver

30
Q

Stain for Borrelia only

A

Giemsa

31
Q

Causative agent of leptospirosis

A

Leptospira interrogans

Elsevier Bacteriology (RVSP) (34 decks)

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