11. Family Neisseriaceae Flashcards
Microscopic appearance of the family Neisseriaceae
Gram-negative diplococci
Oxidase reaction of the family Neisseriaceae
Oxidase positive
Catalase reaction of the family Neisseriaceae
Catalase positive (most)
Neisseria spp. that is catalase negative
Neisseria elongate
MOT of N. gonorrhoeae
Sexually transmitted
Presentation of gonorrhoea in males
Acute urethritis with dysuria and urethral discharge
Presentation of gonorrhoea in females
Colonizes the endocervix causing: Vaginal discharge Dysuria Abdominal pain May lead to pelvic inflammatory disease
Selective media for Neisseria gonorrhoea
Modified Thayer-Martin
Chocolate agar
Incubation requirements of N. gonorrhoea
3-7% CO2
35-37°C
(Humidity important)
Microscopic appearance of N. gonorrhoea
Gram-negative diplococci (some may appear as tetrads)
Colony morphology of N. gonorrhoea on Chocolate agar
Small
Gray
Translucent
Raised colonies
Colony morphology of N. gonorrhoea on blood agar
N. gonorrhoea does not grow on blood agar
Catalase reaction of N. gonorrhoea
Catalase positive
Oxidase reaction of N. gonorrhoea
Oxidase positive
Carbohydrate oxidized by N. gonorrhoea
Glucose
Agar used to determine the sugar oxidized by N. gonorrhoea
Cystine Trypticase agar (CTA)
Specimens for N. meningitidis diagnosis
CSF
Blood
Skin scraping
Most important strains of N. meningitidis clinically
A B C Y W135
Amount of CSF needed for diagnosis of N. meningitidis infection
Greater than 1 mL CSF
Yes/No. Can the CSF specimen for N. meningitidis diagnosis be refrigerated?
No
Microscopic characteristics of N. meningitidis
Gram-negative diplococci found intracellularly and extracellularly
Media used to grow N. meningitidis
Sheep blood agar
Chocolate agar
Thayer-Martin agar
Catalase reaction of N. meningitidis
Catalase positive
Oxidase reaction of N. meningitidis
Oxidase positive
Carbohydrates oxidized by N. meningitidis on CTA
Glucose
Maltose
Emumerate other nonpathogenic Neisseria spp.
N. lactamica
N. flavescens
N. sicca
Oxygen requirements of the family Neisseriaceae
Aerobic