7.1 Analyzing DNA (CQ - exam)

Question 1

action of a restriction endonuclease.

Answer 1

Cleaves double stranded DNA molecule. Recognizes the target sequence. Cuts the strand at the restriction site.

Question 2

Explain why DNA fragments migrate in gel electrophoresis. Which fragments migrate farthest: large or small?

Answer 2

DNA fragments migrate based upon mass and electrical charge. The fragments tend towards the gel’s positive end and smaller fragments migrate more quickly.