1.3 Biochemical Reactions (CQ - Exam)

Question 1

Differentiate between a hydrolysis reaction and a condensation reaction.

Answer 1

Condensation reactions joins 2 molecules by removing water

Hydrolysis reaction breaks a larger molecule into 2 smaller molecules by adding water

Question 2

Meaning of the term enzyme-substrate complex. .

Answer 2

• Enzyme breaks bonds in the substrate and catalyzing the reaction
• The substrate enters into the enzyme at the active site
• When enzyme forms around the substrate’s shape is called an induced fit

Question 3

Clearly explain how an enzyme can take part in a reaction that involves sucrose but not in a reaction that involves maltose.

Answer 3

• Sucrose and maltose look similar in structure but due to the slight shape different, an induced fit cannot occur
• It is a lock and key fit so it will not work, the active sit is a specific shape for a specific substrate

Question 4

Would enzyme denaturation occur at the primary, secondary, or tertiary structure? Explain.

Answer 4

• Not primary
• May effect the other interactions in other stages