5 Kahoot - exam Flashcards

1
Q

Monomer of DNA

A

nucleotide

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2
Q

backbone of DNA

A

phosphate and sugar

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3
Q

pyrimidines

A

cytosine and thymine

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4
Q

number of rings in pyrimidines

A

1

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5
Q

complementary base pairing refers to

A

the arrangement of nitrogenous bases in DNA strands

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6
Q

what are the complementary base pairs in DNA

A

A always binds with T; G always binds with C

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7
Q

How many hydrogen bonds between adenine and thymine?

A

2

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8
Q

Antiparallel means that

A

strands of DNA run in opposite directions

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9
Q

What is attached to the 3’ end of DNA?

A

An exposed hydroxyl group

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10
Q

What is attached to the 5’ end of DNA?

A

Phosphate

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11
Q

How many nucleotides are in one turn of DNA?

A

10 basepairs

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12
Q

What is a genome?

A

An organism’s complete set of hereditary information.

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13
Q

What is a gene?

A

A functional unit of DNA

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14
Q

How is eukaryote DNA different than prokaryote DNA?

A

eukaryote DNA is linear

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15
Q

Where is the DNA found in a prokaryotic cell?

A

Nuceloid Region

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16
Q

Which enzyme controls the twisting of DNA in Prokaryotes?

A

topoisomerase

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17
Q

What is a plasmid?

A

A small loop of DNA that can be copied and shared.

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18
Q

What is a nucleosome?

A

region of DNA wound around histone proteins

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19
Q

In what form is DNA during interphase?

A

chromatin

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20
Q

At what stage of the cell cycle does DNA replication occur?

A

S phase

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21
Q

In order, what are the three main phases of DNA replication?

A

initiation, elongation, termination

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22
Q

What is the replication fork?

A

the region where parental strands of DNA are being unwound

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23
Q

What is a replication bubble

A

A seperated segment of DNA where replication originates

24
Q

Helicase unwinds the DNA by:

A

breaking hydrogen bonds

25
After the DNA is unzipped by DNA helicase, what happens next?
SSBPs stabilize DNA strands so they don't anneal
26
What is the name of the enzyme that relieves the stress on the DNA during unwinding?
Topoisomerase
27
What enzymes adds nucleotides to build a new strand of DNA?
DNA polymerase III
28
What is required before DNA polymerase can add new nucleotides to build the new strand?
RNA primer
29
What end of the DNA strand does DNA Polymerase add nucleotides to?
3'
30
how many primers are required for each leading strand?
1
31
Which strand of DNA is built discontinuously?
lagging
32
tRUE/FALSE: The overall direction of DNA replication in the lagging strand is opposite that of the leading strand
false
33
The overall direction of DNA replication is:
away from the origin of replication
34
Why is the lagging strand built up in a discontinous fashion?
DNA synthesis is in the 5' to 3' direction (adding nuceotides to 3' end)
35
What are the fragments on the lagging strand called?
okazaki
36
What is the enzyme that removes and replaces the RNA primers with DNA nucleotides called?
DNA polymerase I
37
What is the enzyme that forms the phosphodiester bonds between DNA fragments called?
Ligase
38
What occurs in the termination phase of DNA replication?
DNA strands rewind
39
What enzyme is responsible for the proofreading of DNA during replication?
polymerase
40
When does DNA polymerase perform its proofreading function on mispaired nucleotides?
before adding the next nucleotide in the chain
41
DNA polymerase "proofreads" each new DNA strand. Why would DNA need "proofreading?"
The new strand needs to be a perfect copy.
42
An unfixed mistake made during DNA replication is called:
Mutation
43
How does DNA polymerase recognize mismatched base pairs?
3' OH- of mismatched base incorrectly positioned for DNA polymerase
44
Strand slippage causes:
addition or deletion of bases
45
What is the function of an exonuclease?
To correct mistakes made by DNA polymerase in replication
46
How does DNA replication differ in Prokaryotes vs Eukaryotes?
- Prokaryotes have one origin of replication and Eukaryotes have many - Number/types of DNA polymerase - Only Eukaryotes have telomeres
47
What are telomeres?
a repeating sequence of DNA at the end of a chromosome.
48
What is a function of telomeres?
protect the genes on our DNA during replication
49
Why is an RNA primer necessary in DNA replication?
Provides a 3' end that DNA polymerase can add nucleotides to
50
Why is DNA shorter after replication?
RNA primer is bound to the 5' end of the strand.
51
Which enzyme prevents the loss of telomeres during DNA replication?
Telomerase
52
What is cell senescence?
when a cell can no longer divide
53
Why are telomeres considered a biological clock?
Cell division stops after telomeres substantially degrade
54
Hayflick's limit is...
the number of times a normal cell can divide before cell division stops
55
Cancer cells have more telomerase than non cancerous cells.
t