560B Biochem of nutrition Flashcards
T or F: For every amino acid, there is a specific, unique transport system for digestion.
False
T or F: The hemoglobin molecule is more easily saturated with oxygen than is myoglobin, at a given partial pressure of oxygen.
False
The affinity of hemoglobin for oxygen is less than its structural analog myoglobin. Myoglobin, on the other hand, has a significantly higher affinity for oxygen and will, therefore, be much less inclined to release it once it is bound.
T or F: Ammonia formed from muscle amino acid degradation is transferred in the blood in the form of glutamine
True
Thermodynamically speaking, which value most specifically and directly defines whether a biochemical reactions spontaneous or not?
ΔG (gibbs free energy?)
That 3 parts are attached to the central carbon of an amino acid?
a hydrogen atom
a carboxyl group
an amine group
the urea cycle takes place partly in the ____ and partly in the ____
mitochondria, cytosol
During transamination, one product is always?
glutamate
When pyridoxyl phosphate amine is formed, that means that:
transamination is occurring
What are 3 metabolic sources of ammonia?
purines and pyrimidines
the diet
bacterial degradation of urea
the _____ forms of amino acids are incorporated into human protein
L isomer
ionization of the _____ would increase as you went above the pKa, say the body’s pH
carboxyl groups
What is the significance of the pKa as it applies to the body?
- ultimately it can bear greatly upon the way a protein folds
- it means that some R groups of amino acids will never ionize
- both of the above are true
- none of the above are true
not totally sure:
1. ultimately it can bear greatly upon the way a protein folds
From lecture: The electrochemical, oxidative/reductive environment is closely related to local pH, which is a critical factor in the way a protein folds,
(not 3)
With regard to ALT and AST:
- they are two muscle proteins that allow muscle contraction
- they have little clinical diagnostic value in the case of liver damage
- they are used in the oxidative deamination process
- they both require pyridoxyl phosphate (vitamin B-6) as a coenzyme
they both require pyridoxyl phosphate (vitamin B-6) as a coenzyme
Which of the follow is true?
- polypeptides that have similar amino acid sequences and function are analogous
- amino acids within a given protein that do not differ from species to species are defined as invariant, because they are essential to the function of that protein
- insulin is synthesized in zymogen form, but peptide C is not.
- supersecondary motifs and domains are generally not the same thing
- amino acids within a given protein that do not differ from species to species are defined as invariant (never changing), because they are essential to the function of that protein
With regard to the different forms of water in the cell, which is not true?
- water at the surface of a protein can bare on how much of a particular ion binds to that protein
- sodium and potassium both being positive would be expected to bind to a proteins surface to the same degree
- water is responsible, at least in part, for formation of microenvironments within the cell
- the high specific heat of water helps maintain body temperature in cases where some of the heat energy that is produced from a biochemical reaction (and that is not used for driving other biochemical reactions) is given off as heat
- the ATP-requiring sodium/potassium pump only partly explains the partitioning of sodium and potassium outside and inside the cell membrane, respectively
- sodium and potassium both being positive would be expected to bind to a proteins surface to the same degree
Which is correct regarding protein structure?
• there are at least two subunits that are needed to form quaternary structure
• most tertiary structures contain a hydrophobic inner center
• multiple subunits make repair of the protein easier, as less energy is wasted repairing places that are not in need of repair
• all of the above are true
all of the above are true
Which is true regarding protein?
- amino acid pools are small in part because protein is not the main body fuel
- amino acid pools are small in part because there are no proteins and their related enzyme systems designed specifically for amino acid storage
- urea is formed in the liver, because there is a great deal of amino acid degradation there
- all of the above are true
- all of the above are true
True/false: The Km is a measure of the enzyme’s velocity at the substrates concentration that allows the reaction to reach maximum velocity
False
Km= Michaelis constant
defined as the substrate concentration at 1/2 the maximum velocity
True/false: the straight line portion of the Michaelis/Mention represents the initial velocity
True
True false: Regarding enzymes, the sigmoidal shape curve kinetics represents a multi-subunit and/or multi-active site enzyme, which could be subject to both enzyme induction and feedback regulation (inhibition)
True
True/false: A word with which you could easily associate the taut and relaxed state with would be allosterism.
True
allosterism: A change in the activity and conformation of an enzyme resulting from the binding of a compound at a site on the enzyme other than the active binding site
Which statement is true regarding collagen?
- it is composed of two helical polypeptides wound around one another
- it is composed of about one third glycine because that amino acid is small and the collagen is compact
- it has a relatively short half-life compared to other proteins, but longer than that of enzymes
- mutations associated with the synthesis of collagen hardly ever occur
it is composed of about one third glycine because that amino acid is small and the collagen is compact
Regarding sickle cell anemia:
- it arises because of a mutation that inserts a valine to replace glutamine in the beta subunit of the hemoglobin
- electrophoresis of a person’s hemoglobin can identify a patient with sickle cell trait vs. one with full blown sickle cell anemia
- glutamate is replaced with valine, resulting in a hydrophobic protrusion from one of beta subunit finding a hydrophobic pocket of another beta subunit of another hemoglobin, so that the hemoglobins will polymerize with one another
- the polymerization process takes place during the oxygenated state
electrophoresis of a person’s hemoglobin can identify a patient with sickle cell trait vs. one with full blown sickle cell anemia
Regarding antibody structure:
- the variable region is the only part of the structure that differs among antibodies,thus being theromynamically efficient regarding their synthesis
- the antibody fits with its antigen according to the lock and key theory
- the C regions of an antibody vary considerably from antibody type to antibody type
- all of the above are true
the variable region is the only part of the structure that differs among antibodies,thus being theromynamically efficient regarding their synthesis
Regarding Insulin:
- it is composed of a single polypeptide chain of 51 amino acids
- it is not synthesized in zymogen form, because it is not a potentially destructive enzyme
- its structure is stabilized by disulfide bonds
- it is synthesized as an inactive precursor known as peptide
its structure is stabilized by disulfide bonds
The best theoretical cure for sickle cell anemia would be:
- bone marrow transplants
- gene replacement therapy
- transfusions
- all of the above are equally viable
gene replacement therapy
Which of the follow are true regarding enzymes?
- in non-competitive inhibition the Vmax of the enzyme without an inhibitor would be reached
- in competitive inhibition, the Vmax of the enzyme curve can never be reached by adding more and more substrate, because the inhibition is at the active site
- a Lineweaver/Burke plot will show all the information that a Michaelia/Menton plot will show
- none of the above are true
a Lineweaver/Burke plot will show all the information that a Michaelia/Menton plot will show
Regarding turnover number of an enzyme:
- carbonic anhydrase is one of the fastest enzymes in the cell
- digestive enzymes would tend to have lower turnover numbers, because the digestive process must not proceed too quickly, or absorption of nutrients would be theoretically be impaired
- as long as the concentration of active sites of an enzyme are known, then the Vmax reveals the turnover number of that enzyme
- all of the above are true
- all of the above are true
Regarding enzymes:
• the activity of the enzyme can be affected by what’s associated with its domains
• lyases catalyze the formation of bonds, such as C-O and C-S, accompanied by the use of ATP
• enzymes alter the equilibrium of a reaction
• allosteric enzymes show a hyperbolic-shape curve
• all of the above are true
the activity of the enzyme can be affected by what’s associated with its domains
Regarding enzymes:
- a whole metabolic pathway can be controlled by controlling the activity of a key enzyme that is involved in that pathway
- a transition state is one where the enzyme is complexed to a very high-energy level, relatively unstable intermediate
- for thermal effects upon enzymes, a bell shaped curve would be expected
- all of the above are true
- all of the above are true
Which is true?
- myoglobin can be considered a storage protein in the liver
- myoglobin is identical to either the alpha or the beta subunit of hemoglobin
- myoglobin shows tertiary structure
- all of the above are true
myoglobin shows tertiary structure
True/False: protein phosphateases can remove phosphate groups from enzymes
True
True/False: Internal respiration centers on the fact that oxygen is the final electron acceptor in the electron transport system.
True
True/False: The electron transport system is located within the mitochondrial matrix.
False
it is found in the inner mitochondrial membrane
True/False: The primary reason the Kreb’s cycle turns in one direction is because of the side pathways that generate other products from the cycle.
False: most of the reactions are actually reversible
True/False: When the ratio of NAD+/NADH is high, the inter-conversion of lactate and pyruvate favors lactate
False: pyruvate can be converted to lactate.
This occurs when there is insufficient oxygen in the tissue and when there is a high NADH/NAD+ ratio, both occurring in heavily-exercising muscle, for example
True/false: A high insulin/glucagon ratio favors glycogenolysis
False: Fructose 2,6-bisphosphate strongly stimulates phosphofructokinase and inhibits fructose 1,6-bisphosphatase. Hence, glycolysis is accelerated and gluconeogenesis is diminished in the fed state.